Syntheses of apatite via Ca complexes of amino acids involved innon-collagen protein

通过参与非胶原蛋白的氨基酸的 Ca 络合物合成磷灰石

• 批准号: 22550183
• 负责人: SATO Mitsunobu
• 金额: $ 3.08万
• 依托单位: Kogakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22550183/
• 关键词: 生体材料; 無機工業化学; 再生医学; 生体分子; ナノバイオ

The apatite powders having low crystallinity were prepared using Ca complexes of amino acid ligands. The strength of the matrix, apatite crystallinity and distribution affected the regeneration ability of the 3 dimensional porous scaffolds with the prepared apatites and a biodegradable polymer (PLGA). The aqueous-solution coating (ASC) method was newly developed to afford a position and shape model of apatite in scaffolds. The cell attachment and expansion of osteocalcin on the apatite f ilms having characteristic network structure by ASC method were also examined. Based on theseresults, scaffold fabrication by apatite deposition directly in gelatin was newly achieved through the complexes of amino acids involved in non-collagen protein.

采用氨基酸配体的钙配合物制备了低结晶度的磷灰石粉体。基质的强度、磷灰石的结晶度和分布影响了制备的磷灰石和生物可降解聚合物（PLGA）的三维多孔支架的再生能力。采用水溶液包覆法（ASC）建立了磷灰石在支架中的位置和形状模型。用ASC法观察了骨钙素在具有典型网状结构的磷灰石膜上的细胞附着和扩增。在此基础上，利用非胶原蛋白氨基酸的络合物，实现了在明胶中直接沉积磷灰石制备支架的新方法。

项目成果

Hydroxyapatite coating for titanium fibre mesh scaffold enhances osteoblast activity and bone tissue formation

• DOI: 10.1016/j.ijom.2011.12.035
• 发表时间: 2012-10-01
• 期刊: INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
• 影响因子: 2.4
• 作者: Hirota, Makoto;Hayakawa, Tohru;Tohnai, Iwai
• 通讯作者: Tohnai, Iwai

Thin Hydroxyapatite Coating on Porous Beta-Tricalcium Phosphate (β-TCP) Enhances Osteoblast Function Activity

多孔 β-磷酸三钙 (β-TCP) 上的薄羟基磷灰石涂层可增强成骨细胞功能活性

• 发表时间: 2012
• 期刊: Journal of Hard Tissue Biology
• 影响因子: 0.4
• 作者: Yuka Monden;Makoto Hirota;Tohru Hayakawa;Mitsunobu Sato;Shogo Murata;Yuki Sato;Jiro Maegawa;Iwai Tohnai
• 通讯作者: Iwai Tohnai

Coordination Engineering Laboratory

协调工程实验室

硬組織再生用スキャフォールドとアパタイト薄膜付与材料の形成と応用

硬组织再生支架和磷灰石薄膜赋予材料的形成及应用

• 发表时间: 2013
• 作者: H. Nakamura;Y. Obukuro;K. Obata,S. Matsushima;M. Arai;K. Kobayashi;伊崎昌伸;佐藤光史
• 通讯作者: 佐藤光史

In vitro examination of apatite/PLGA composite including low crystallinity apatites prepared from Ca complexes of IDA and Asp

磷灰石/PLGA 复合材料（包括由 IDA 和 Asp 的 Ca 复合物制备的低结晶度磷灰石）的体外检查

• 发表时间: 2011
• 作者: Chihiro Mochizuki;Tohru Hayakawa;Shen-Guo Wang;Mitsunobu Sato
• 通讯作者: Mitsunobu Sato