Development of Purification Process of Plasmid DNA for Gene Therapy Using Affinity Membrane Filtration

近年来，利用质粒DNA作为载体进行基因治疗或DNA疫苗接种已成为治疗疾病的新方法。对于这样的临床应用，越来越需要纯化工艺来生产药用级质粒DNA。本文综述了亲和膜过滤技术在质粒DNA纯化和配体寻找中的应用。除了质粒DNA与配体结合过程中的选择性行为和结合质粒DNA的洗脱过程外，还研究了膜污染引起的通量下降行为。琼脂糖凝胶分析证实了该方法纯化质粒DNA的有效性。

Recently, the use of plasmid DNA as vector for gene therapy or DNA vaccination has become increasingly popular as novel ways of dealing with disease. For such clinical applications, there is a growing need for purification processes to produce pharmaceutical-grade plasmid DNA. In the present study, the application of affinity membrane filtration to plasmid DNA purification and the search of ligand are examined. In addition to the selectivity behaviors in the binding process of plasmid DNA to ligand and the elution process of the bound plasmid DNA, the flux decline behaviors caused by the membrane fouling are also investigated in this system. The agarose gel analysis confirmed usefulness of this method for plasmid DNA purification.