Application of gene targeting by homologous recombination as a biothecnological tool for rice functional genomics.

应用同源重组基因打靶作为水稻功能基因组学的生物理论工具。

• 批准号: 22570049
• 负责人: JOHZUKA Yasuyo
• 金额: $ 3万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570049/
• 关键词: 遺伝子ターゲティング; 相同組換え; 変異導入; 植物; イネ; 形質転換; バイオテクノロジー; 遺伝子; バイオテクノロジ

Rice, an important food, is the staple grain for one-half of the world’s population. We have succeeded in the development of a gene-targeting method which involves the genetic modification of rice DNA. Aiming at wide use of targeting of a higher plant, we developed making suitable recombination enzyme (λRed system) , and making targeting frequency increase. The vectors for assay for measuring targeting frequency were also developed.

大米是一种重要的食物，是世界上一半人口的主粮。我们已经成功地开发出了一种涉及水稻DNA遗传修饰的基因打靶方法。针对高等植物靶向的广泛应用，我们研制了合适的重组酶(λ红系)，使靶向频率提高。并建立了测定靶向频率的检测载体。

项目成果

The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea

• DOI: 10.1007/s10265-010-0369-7
• 发表时间: 2011-03-01
• 期刊: JOURNAL OF PLANT RESEARCH
• 影响因子: 2.8
• 作者: Johzuka-Hisatomi, Yasuyo;Noguchi, Hiroshi;Iida, Shigeru
• 通讯作者: Iida, Shigeru