functional evolution of nebulin superfamily in vertebrates

点击翻译按钮获取中文摘要

Nebulin superfamily proteins such as lasp, lasp-1, lasp-2, nebulin, nebulette, and N-RAP have similar domain structures (nebulin repeats, LIM domain and SH3 domain) and are thought to be generated from the same ancestral gene. This study tried to reveal their functional evolution in nerve and muscle tissues in vertebrates. We found tissue distribution of lasp-1 and lasp-2 were different in zebra fish. Localization of lasp-1 and lasp-2 were different in primary neurons of chicken and rat and siRNA of lasp-2 and dominant negative constructs of lasp-2 resulted morphological abnormality in filopodia in the primary neurons. Lasp-2 play roles in vesicle transport, cell-substrate attachment and peripheral astrocyte process in glial cells of chicken and rat.