Structural analysis of supra-biomolecules by using ion mobility mass spectrometry

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Structural study of supra-biomoleculeswere executed by using ion mobility mass spectrometry (IM-MS), which provides not only of masses but also of collision cross-sections of the supra-biomolecules. Since IM-MS is applicable to theprotein complexes that are difficult to crystallize, it was possible to analyze theirstability and conformational alteration by IM-MS. In the present study, a calculation method of collision cross sectionsof dummy residue models for noncrystallizable supra-biomolecules was established.In addition, it was proved by IM-MS and molecular dynamics (MD) simulation that behaviors of the folded and unfolded regions in the gas phase were remarkably varied.