Coordination mechanism of multiple motor proteins : a DNA-protein hybrid nanosystem study

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The motor protein kinesin drive vesicle transport in the cell using energy derived from ATP. However, how multiple kinesin are coordinated each other remains elusive. Here we created a hybrid nanomachine(DNA-Tile-kinesin) using DNA-Tile as the skeletal structure and kinesin as the functional module. Single molecule imaging of DNA-Tile-kinesin hybrid allowed us to evaluate the effects of the number of molecules involved in the transport and nanometer changes in the distance between multiple kinesins on motility. Our results show that number of kinesin participating in the transport affect on run length but not on velocity. These results indicate the power of our approach toward the understanding of coordination mechanism of motor proteins.