Regulatory mechanism of Topoisomerase1 by small RNA molecules which leads genomic instability

小RNA分子对拓扑异构酶1的调节机制导致基因组不稳定

• 批准号: 22570169
• 负责人: KOBAYASHI Maki
• 金额: $ 3万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570169/
• 关键词: non-B DNA; AID; 免疫グロブリン; miRNA; 免疫グロブリン遺伝子; DNA切断; Non-B DNA; Topoisomerase1; RNA編集; 翻訳調節

The molecular mechanism which suppresses Topoisomerase1 (Top1) translation in AID-dependent manner was analyzed. Involvement of miRNA, HuR and Ago2, and Ago2-binding sequence in Top1 mRNA have been realized. The putative miRNA which binds to Top1 mRNA AID-dependently will be identified very soon.

分析了艾滋病依赖性抑制拓扑异构酶1（Top1）翻译的分子机制。已经实现了miRNA、HuR和Ago2以及Ago2结合序列在Top1 mRNA中的参与。与Top1 mRNA呈AID依赖性结合的miRNA将很快被鉴定出来。

项目成果

Begum and Tasuku Honjo. AID-induced decrease in topoisomerase1 induces DNA structural alteration and DNA cleavage for class switch r e c o m b i n a t i o n

贝古姆和本庄佑。

• 发表时间: 2010
• 作者: Maki Kobayashi;Masatoshi Aida;Hitoshi Nagaoka;Nasim A
• 通讯作者: Nasim A

Activation-induced cytidine deaminase regulates Topoisomerase1 protein by miRNA during immnoglobulin gene recombination

免疫球蛋白基因重组过程中激活诱导的胞苷脱氨酶通过 miRNA 调节拓扑异构酶 1 蛋白

• 发表时间: 2013
• 作者: 松浦祥悟;他;Maki Kobayashi et al
• 通讯作者: Maki Kobayashi et al

AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination

• DOI: 10.1073/pnas.0911879106
• 发表时间: 2009-12-29
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Kobayashi, Maki;Aida, Masatoshi;Honjo, Tasuku
• 通讯作者: Honjo, Tasuku

AID-induced Topoisomerase1 reduction regulates somatic hypermutation frequency

AID 诱导的拓扑异构酶 1 减少调节体细胞超突变频率

• 发表时间: 2011
• 作者: Maki Kobayashi;Zahra Sabouri;Masatoshi Aida;Yoko Kitawaki;and Tasuku Honjo
• 通讯作者: and Tasuku Honjo

Epigenetic regulation of S region chromatin is essential to AID-induced S region DNA cleavage by topoisomerase 1.

S 区染色质的表观遗传调控对于 AID 诱导的拓扑异构酶 1 切割 S 区 DNA 至关重要。

• 发表时间: 2010
• 作者: Maki Kobayashi;Masatoshi Aida;Andre Stanlie;Nasim A.Begum;Hitoshi Nagaoka;Tasuku Honjo.
• 通讯作者: Tasuku Honjo.