Transcriptional regulation of HSF1 by RPA

RPA 对 HSF1 的转录调控

• 批准号: 22570171
• 负责人: FUJIMOTO Mitsuaki
• 金额: $ 2.91万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570171/
• 关键词: HSF1; RPA; FACT; がん; 転写; RNA Polymerase II

Heat shock factor 1 (HSF1) plays roles in control and stressed conditions by gaining access to target elements, but mechanisms of HSF1 access are not well known in mammalian cells. I found the physical interaction between the wing motif of human HSF1 and replication protein A (RPA), which is involved in DNA metabolism. Depletion of RPA1 abolishes HSF1 access to the promoter of HSP70 in unstressed condition and delays its rapid activation in response to heat shock. The HSF1-RPA complex leads to preloading of RNA polymerase II and opens the chromatin structure by recruiting a histone chaperone, FACT. Furthermore, this interaction is required for melanoma cell proliferation.

热休克因子1（HSF 1）通过接近靶元件在控制和应激条件下发挥作用，但HSF 1接近哺乳动物细胞的机制尚不清楚。我发现了人类HSF 1的翼基序和复制蛋白A（RPA）之间的物理相互作用，这是参与DNA代谢。RPA 1的缺失在非应激条件下废除了HSF 1对HSP 70启动子的访问，并延迟了其响应热休克的快速激活。HSF 1-RPA复合物导致RNA聚合酶II的预加载，并通过招募组蛋白伴侣FACT打开染色质结构。此外，这种相互作用是黑素瘤细胞增殖所必需的。

项目成果

Impaired hippocampal spinogenesis and neurogenesis and altered affective behavior in mice lacking heat shock factor 1

• DOI: 10.1073/pnas.1016424108
• 发表时间: 2011-01-25
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Uchida, Shusaku;Hara, Kumiko;Watanabe, Yoshifumi
• 通讯作者: Watanabe, Yoshifumi

Glutamine Protects Intestinal Barrier Function of Colon Epithelial Cells from Ethanol by Modulating Hsp70 Expression

• DOI: 10.1159/000345930
• 发表时间: 2013-01-01
• 期刊: PHARMACOLOGY
• 影响因子: 3.1
• 作者: Akagi, Reiko;Ohno, Michiko;Inouye, Sachiye
• 通讯作者: Inouye, Sachiye

Assists HSF1 Access to Nucleosomal DNA by Recruiting Histone Chaperone FACT

通过招募组蛋白伴侣协助 HSF1 获取核小体 DNA 事实

• 发表时间: 2012
• 期刊: Molecular Cell
• 影响因子: 16
• 作者: L. BlackmanM.;M. Arikawa;S. Yamada;T. Suzaki and A. Hardham;安藤元紀・洲崎敏伸;T. Suzaki;洲崎敏伸;Reiko Akagi;MItsuaki Fujimoto
• 通讯作者: MItsuaki Fujimoto

HSF1-RPA複合体はHSP70遺伝子へのRNAポリメラーゼのプリロードに必要である

HSF1-RPA 复合物是 RNA 聚合酶预加载到 HSP70 基因上所必需的

• 发表时间: 2011
• 作者: 藤本充章;高木栄一;瀧井良祐;Prakasam Ramachandran;譚克;林田直樹;家村俊一郎;夏目 徹;中井 彰
• 通讯作者: 中井 彰