Investigation of GPCRs interacting with RAMPs an functional analysis of their complex.

(I)我开发了一种系统，用于鉴定与给定靶蛋白相互作用的蛋白质，方法是将光反应性氨基酸结合到靶蛋白中，在体内与相互作用的蛋白质进行光交联，并通过比较定量质谱分析鉴定由此捕获的蛋白质。一种新型的长链光反应氨基酸也被成功地结合到蛋白质中，从而实现了蛋白质的大范围光交联。（II）通过x射线晶体结构分析和位点特异性光交联实验，展示了RAMP和降钙素受体样受体在活细胞膜上如何相互作用。

(I) I developed a system for the identification of proteins interacting with a given target protein, by site-specific incorporation of a photo-reactive amino acid into the target protein, in vivo photo-cross-linking to interacting proteins, and identification of thus captured proteins by comparative quantitative mass spectrometry analysis. A novel long chain photoreactive amino acid was alsosuccessfully incorporated into proteins, which enables wide-range photo-cross-linking of proteins.(II) I showed how RAMP and calcitonin receptor-like receptor interact with each other on the membrane of living cells by X-ray crystal structure analysis and site-specific photo-cross-linking assay.