MembraneinducedalterationsofstructureandsignaltransductionmechanisminthePIP3-bindingdomains

膜诱导 PIP3 结合域结构和信号转导机制的改变

• 批准号: 22570191
• 负责人: TUZI Satoru
• 金额: $ 2.91万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570191/
• 关键词: 細胞内情報伝達; 固体NMR 分光法; PH ドメイン; 分光法; シグナル伝達; 膜表在性蛋白質; 脂質二重膜; 生物物理; PHドメイン; SWAP-70; PIP3; 固体NMR; 膜結合性蛋白質; 二次構造

Conformations of the pleckstrin homology (PH) domain in solutionand at the membrane surface were examined by using solid-state NMR, circular dichroism(CD) and fluorescence spectroscopy. The PH domains form one of the largest domainfamilies known to regulate membrane association of host proteins. Drastic conformationalalterations including a transition of the secondary structure were detected for themembrane-bound PH domain of a signal transduction protein SWAP-70. Theseconformational alterations induced at the membrane surface are suggested to regulatefunctions of SWAP-70, such as intracellular relocalization in response to the cellularsignaling.

用固体核磁共振、圆二色谱和荧光光谱研究了普列克底物蛋白（PH）结构域在溶液中和膜表面的构象。PH结构域是已知调节宿主蛋白膜结合的最大结构域家族之一。检测到信号转导蛋白SWAP-70的膜结合PH结构域发生了剧烈的构象变化，包括二级结构的转变。在膜表面诱导的构象改变被认为调节SWAP-70的功能，例如响应细胞信号的细胞内重新定位。

项目成果

Molecular Biophysics II

分子生物物理学II

Conformationalalterations of the pleckstrin homologydomains at the lipid bilayer surface

脂质双层表面普莱克斯特林同源结构域的构象改变

• 发表时间: 2010
• 作者: S. Tuzi;N. Tokuda;K. Kawai;Y. Fukui(National Health Research Institutes,Taiwan);H. Yagisawa
• 通讯作者: H. Yagisawa

Membrane-induced alteration of the secondary structure in the SWAP-70 pleckstrin homology domain.

• DOI: 10.1093/jb/mvr146
• 发表时间: 2012-04
• 期刊: Journal of biochemistry
• 影响因子: 2.7
• 作者: N. Tokuda;K. Kawai;Young-Ho Lee;T. Ikegami;S. Yamaguchi;H. Yagisawa;Y. Fukui;S. Tuzi

脂質二重膜上におけるDef6 PH ドメインの高次構造の解析

脂质双层膜上 Def6 PH 结构域的高阶结构分析

• 发表时间: 2012
• 作者: N. Tokuda;M. Tanio;K. Nishimura;T. Kohno;D. Yokogawa;T. Ikegami;S. Tuzi
• 通讯作者: S. Tuzi

A solid-state NMR study of structural alteration and function of the PH domain induced at the lipid bilayer surface

脂质双层表面诱导的 PH 结构域的结构改变和功能的固态 NMR 研究

• 发表时间: 2011
• 作者: (Takeshi Mizuno);Miwa Higaki;Asami Shibuya;Fumio Hanaoka;Naoko Imamoto;徳田尚美;安堵城悟;永渕昭良;Naomi Tokuda
• 通讯作者: Naomi Tokuda