Transcriptome analysis of wheat and Fusarium graminearum for Fusarium head blight resistance in wheat.

小麦和禾谷镰刀菌转录组分析对小麦赤霉病的抗性。

• 批准号: 22580009
• 负责人: BAN Tomohiro
• 金额: $ 2.91万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580009/
• 关键词: 赤かび病抵抗性; 遺伝子発現; マイコトキシン; QTL; マイクロアレイ; 植物育種・遺伝; 抵抗性・耐性; コムギ; フザリウム菌; クロストーク

Fusarium graminearum causes a critical wheat disease Fsarium head blight (FHB) produces mycotoxyn such as DON and NIV with highly expression of Tri genes during their initial infection to wheat spikes. We analyzed the three Tri genes expression (Tri5, Tri6 and Tri10) by RT-PCR in the infected spikes of five wheat varieties with different level of HB resistance. The Tri genes expression was influenced by the wheat genotype which was highly induced resistance varieties in the early stage of infection and no induction in the susceptible varieties along with their FHB resistance level. It revealed that FHB resistance wheat genotype has active defense mechanism to cross-talk with Fusarium gene expressions.

禾谷镰刀菌 (Fusarium graminearum) 引起严重的小麦病害 赤霉病 (FHB) 会产生霉菌毒素，例如 DON 和 NIV，并在最初感染小麦穗时高度表达 Tri 基因。我们通过 RT-PCR 分析了 5 个不同 HB 抗性水平的小麦品种受感染穗中的 3 个 Tri 基因（Tri5、Tri6 和 Tri10）的表达。 Tri基因的表达受小麦基因型的影响，小麦基因型在感染初期是高度诱导的抗性品种，而感病品种则没有诱导及其FHB抗性水平。研究表明，FHB抗性小麦基因型具有与镰刀菌基因表达串扰的主动防御机制。

项目成果

EvoTree; Software for easily drawing the pedigree chart with varietal characteristics

进化树；

• 发表时间: 2011
• 期刊: Breeding Research
• 作者: Akane Takesaki;Tomohiro Ban;Masumi Katsuta
• 通讯作者: Masumi Katsuta

Isolation of wheat multidrug resistance-associated protein gene(TaMRP1), a possible candidate of the multiple traits QTL for the Fusarium head blight resistance.

小麦多药抗性相关蛋白基因（TaMRP1）的分离，可能是抗赤霉病多性状QTL的候选基因。

• 发表时间: 2010
• 作者: Kikuchi R;Niwa S;Ogawa T;Handa H;Ban T
• 通讯作者: Ban T

Genomic characterization of Afghanistan wheat landraces

阿富汗小麦地方品种的基因组特征

• 发表时间: 2014
• 作者: Zenta Nishio;Kanenori Takata;Miwako Ito;Masahiko Tanio;Tadashi Tabiki;Hiroaki Yamauchi;and Tomohiro Ban;Hiroaki Matsuhira;Tomohiro Ban and Alagu Manickavelu
• 通讯作者: Tomohiro Ban and Alagu Manickavelu

Contribution of TaMRP-D1 allele on Qfhs.kibr-2DS in wheat for FHB resistance

TaMRP-D1 等位基因对小麦 Qfhs.kibr-2DS 对 FHB 抗性的贡献

• 发表时间: 2012
• 作者: Niwa S;Kikuchi R;Kubo K;Lewis J;Nitta M;Nasuda S;Handa H;Ban T
• 通讯作者: Ban T

Characterization of a wheat transcription factor, TaWRKY45, and its effect on Fusarium head blight resistance in transgenic wheat plants

• DOI: 10.1270/jsbbs.61.121
• 发表时间: 2011-06-01
• 期刊: BREEDING SCIENCE
• 影响因子: 2.4
• 作者: Bahrini, Insaf;Sugisawa, Motoki;Handa, Hirokazu
• 通讯作者: Handa, Hirokazu