The causes for the appearance of pest species that showed no effect for mating disruption, and introduction of molecular identification method of plusiine species

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We found 8 plusiine species at the lettuce field of Nagano during the study period. Although identification for larval stage has been difficult, we applied a molecular identification method by Multiplex PCR. So, we showed Autographa nigrisigna is dominant species in the lettuce field of Nagano Prefecture. We also examined the cause for the appearance of A. nigrisigna. We showed that the disturbance effect on A. nigrisigna is insufficient. And the components of the mating disruption agent for other pest species is inhibit the disturbance effect for A. nigrisigna. We also showed mated female adults of A. nigrisigna are flying into the field from the outside of the area.