Characterization of organic nitrogen from cattle manure compost based on the molecular weight distribution

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Hot water extractable organic nitrogen from cattle manure compost and from soil applied with the compost were separated by size-exclusion HPLC and detected by chemiluminescent nitrogen detection (CLND) and spectroscopic approaches. It is revealed that the organic nitrogen is composed of three different categories; (1) biomolecule (>100kDa), (2) humic acid like material (100-20kDa), and fulvic acid like material (<20kDa).