Gene expression mechanism by LysR-type transcriptional regulator and basis for self-recognition

LysR型转录调控因子（LTTR）是细菌转录调控因子中最大的家族之一，参与多种基因的表达，如氨基酸生物合成、芳香族化合物降解和毒力因子合成等。本研究从三维结构上揭示了LTTR与DNA结合和诱导物识别的重要氨基酸，建立了LTTR与调控启动子共结晶的蛋白质和DNA纯化方法，为阐明LTTR转录激活机制奠定了基础。

The LysR-type transcriptional regulators (LTTRs) comprise one of the largest families of bacterial transcriptional regulators, involved in expression of many genes of diverse roles such as amino acid biosynthesis, degradation of aromatic compounds, and virulence factor synthesis. This study revealed important amino acids for DNA binding and inducer recognition by LTTR in relation to three dimensional structure, and established methods for purifying protein and DNA for co-crystallization of LTTR and the regulated promoter, which together will lead to elucidation of mechanism of transcriptional activation by LTTRs.