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Expressional and functional characterization of the novel transporter protein related to thiocyanate ion uptake

Expressional and functional characterization of the novel transporter protein related to thiocyanate ion uptake
与硫氰酸根离子摄取相关的新型转运蛋白的表达和功能表征
批准号:
22580082
负责人:
ARAKAWA Takatoshi
金额:
$2.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012

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中文摘要
翻译
硫氰酸盐氢化酶(SCNase)操纵子在编码SCNase各亚基的基因下游含有四个未知基因。目前的研究目的是利用细菌和酵母重组表达系统对基因产物进行实质性表征。到目前为止,推定的核苷酸结合结构域以可溶性蛋白的形式大量表达,而其他三种产物的表达水平较低。
英文摘要
Thiocyanate hydrorase (SCNase) operon contains four unidentified genes at downstream of the ones encoding the respective subunits of SCNase. The current research aimed at substantial characterization of the gene products using bacterial and yeast recombinant expression system. To date, while putative nucleotide binding domainexpressed as a soluble protein at considerable amount, the other three products yielded at lower level.
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DOI: 10.1016/j.jbiosc.2013.01.013
发表时间: 2013-07-01
期刊: JOURNAL OF BIOSCIENCE AND BIOENGINEERING
影响因子: 2.8
作者: [Yamanaka, Yasuaki, Arakawa, Takatoshi, Odaka, Masafumi]
通讯作者: Odaka, Masafumi
Studies on the Construction of Biocatalysts with the Structural Framework of GPCR
  • 批准号:
    26660289
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.5万
  • 财政年份:
    2014
  • 负责人:
    ARAKAWA Takatoshi
  • 依托单位:
海外基金