Structural determination and functional elucidation of novel thiolic glycoconjugates which can form microtube structures

可形成微管结构的新型硫醇糖缀合物的结构测定和功能阐明

• 批准号: 22580117
• 负责人: TAKEDA Minoru
• 金额: $ 3万
• 依托单位: Yokohama National University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580117/
• 关键词: 天然物質化学; Sphaerotilus natans; 鞘; チオペプチドグリカン; 蛍光標識化; 粘質性多糖; 立体構造予測; Leptothrix cholodnii; 鞘新生; マンガン酸化; マイクロカルチャー; 複合糖質; 構造決定; 多糖リアーゼ; Paenibacillus koleovorans

Terminal elongation of microtubes (sheaths) which are extracellulary produced by filamentous ion-oxidizing bacteria, Sphaerotilus natans and Leptothrix cholodnii, was confirmed by fluorescent microscopy. The sheaths were revealedto be commonly assembled from thiolic glycoconjugates composed of amphoteric glycan coreand dipeptide side chain containing glycine and cysteine. Considering their uniquechemical structures, the sheath-forming thiolic glycoconjugates were proposed to becollectively termed thiopeptidoglucan.

荧光显微镜证实了丝状离子氧化菌（Sphaerotilus natans）和钩螺旋体（Leptothrix cholodnii）在细胞外产生的微管（鞘）末端伸长。这些鞘通常由两性聚糖核和含有甘氨酸和半胱氨酸的二肽侧链组成的硫代糖缀合物组装而成。考虑到其独特的化学结构，建议将这些成鞘的硫代糖缀合物统称为硫代肽葡聚糖。

项目成果

A Spatial Relationship between Sheath Elongation and Cell Proliferation in Sphaerotilus natans

球孢菌鞘伸长与细胞增殖的空间关系

• DOI: 10.1271/bbb.120616
• 发表时间: 2012
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: M. Takeda;Takuto Umezu;Yuta Kawasaki;S. Shimura;Keiko Kondo;J. Koizumi
• 通讯作者: J. Koizumi

Conformational analysis of an extracellular polysaccharide produced by Sphaerotilus natans

• DOI: 10.1016/j.carres.2012.07.023
• 发表时间: 2012-10-01
• 期刊: CARBOHYDRATE RESEARCH
• 影响因子: 3.1
• 作者: Kondo, Keiko;Takeda, Minoru;Ueda, Kazuyoshi
• 通讯作者: Ueda, Kazuyoshi

Patterns of sheath elongation, cell proliferation, and manganese(II) oxidation in Leptothrix cholodnii

• DOI: 10.1007/s00203-012-0801-6
• 发表时间: 2012-03
• 期刊: Archives of Microbiology
• 影响因子: 2.8
• 作者: M. Takeda;Yuta Kawasaki;Takuto Umezu;S. Shimura;M. Hasegawa;J. Koizumi

Microtube-forming glycoconjugate of bacterial origin

细菌来源的微管形成糖复合物

• 发表时间: 2010
• 作者: Yamada M;Kondo K;Kawasaki Y;Umezu T;Takeda M;Koizumi J
• 通讯作者: Koizumi J

Study of a novel glycoconjugate, thiopeptidoglycan, and a novel polysaccharide lyase, thiopeptidoglycan lyase

• DOI: 10.1016/j.ijbiomac.2010.11.009
• 发表时间: 2011-03-01
• 期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
• 影响因子: 8.2
• 作者: Kondo, Keiko;Takeda, Minoru;Katahira, Masato
• 通讯作者: Katahira, Masato