Structural changes accompanying mitigation of the allergic action ofovomucoid after passing an electric current

通过电流后伴随卵类粘蛋白过敏作用减轻的结构变化

• 批准号: 22580138
• 负责人: MATSUMOTO Tomoaki
• 金额: $ 2.58万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580138/
• 关键词: 卵アレルギー; 卵アレルゲン; 低アレルゲン化; オボムコイド; 直流電流; プロテオミクス; 蛋白高次構造; 食物アレルギー; 通電処理; 低アレルゲン食品; 質量分析; 鶏卵アレルギー; 卵白オボムコイド; 卵白アレルゲン; 蛋白質量分析

Ovomucoid is the most prominent allergen causing hen’s eggallergy, containing S-S bonds that may be responsible for its allergic action. Theallergic action of this protein has been reduced after electrolysis on both the anodeand the cathode sides when evaluated by mean of skin prick. The results for modificationsof ovomucoid after electrolysis showed two distinct peptide fragments （57E-63K、123H-128R）had lost with matrix-associated laser desorption/ionization time-of-flight massspectrometry. Although the total free SH groups in ovomucoid were increased on thecathode side, the regions for breaking S-S bonds have not been determined in this study

卵泡样蛋白是引起母鸡鸡蛋过敏的最主要的过敏原，含有S-S键，可能是其过敏作用的原因。经皮肤刺痛评价，该蛋白在阳极和阴极两侧电解后的过敏作用均有所降低。通过基质相关激光解吸/电离飞行时间质谱分析，电解后卵泡样蛋白的修饰结果显示，两个不同的肽片段（57E-63K, 123H-128R）丢失了。虽然卵泡样液中游离SH基团在阴极侧增加，但本研究并未确定S-S键断裂的区域

项目成果

Mitigation of the allergicaction of ovomucoid with electricalenergy

用电能减轻卵类粘蛋白的过敏反应

• 发表时间: 2012
• 作者: Tamura H.;et al;梶間未葵（研究代表者加治屋は2番目）;辻田隆廣;Matsumoto T
• 通讯作者: Matsumoto T

Mitigation of the allergic activity of ovomucoid by electrolysis

通过电解减轻卵类粘蛋白的过敏活性

• 发表时间: 2012
• 作者: S. Kano^1;K. Maeyama^2;Y. Wang^2;A. Kondo^2;T. Furumoto^;H. Fukui^and H. Tamura;中村宜督;松本知明
• 通讯作者: 松本知明