Elucidation of the mechanisms for systemic plant immunity induced in the Japanese birch plantlet treated with a new dicarboxylic acid

用水杨酸（SA）或壬二酸（AA）对3月龄的白桦幼苗进行处理。处理2天后，从植株中提取蛋白质，通过双向电泳分离，然后检测处理特异性蛋白质。对这些蛋白的分析结果表明，从SA和AA处理中分别鉴定出11种和8种蛋白。前者参与能量产生、代谢和蛋白质合成，后者参与光呼吸代谢、糖代谢和抗坏血酸生物合成、脂肪酸降解、光合作用、光系统I、防御反应和分子伴侣。综上所述，尽管SA和AA的诱导机制不同，但它们都激活了白桦幼苗的初级代谢和能量产生，从而诱导了白桦幼苗的全身免疫。

Salicylic acid (SA) or azelaic acid (AA) were administered to3-month-old Japanese birch plantlets. Two days after the treatments, proteins were extracted from the plantlets, separated by two-dimensional electrophoresis, and then the treatment-specific proteins were detected. As the results of analyses of these proteins, 11 and 8 kinds of proteins were identified from SA and AA treatments, respectively. The formers were involved in energy production, metabolisms, and protein syntheses, while the latters in photorespiration metabolism, sugar metabolism and ascorbic acid biosynthesis, fatty acid degradation, photosynthesis, photosystem I, defensive response, and molecular chaperone. Based on the obtained results, it was clarified that both SA and AA activate primary metabolism and energy production to induce systemic plant immunity in Japanese birch plantlet, although the induction mechanisms are different between them.