Clarifying the mechanism on systemic acquired resistance in Japanese birch plants infected with a canker-rot fungus, Inonotus obliquus

阐明感染溃疡病真菌桦褐孔菌的日本桦树系统获得性抗性的机制

• 批准号: 19580162
• 负责人: YOKOTA Shinso
• 金额: $ 1.91万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580162/
• 关键词: カバノアナタケ; シラカンバ; プロテオーム; 樹病; サリチル酸; 全身獲得抵抗性; フェノール性化合物; ペルオキシダーゼ; non-expresser of pathogenesis related 1; oxygen evolving enhancer protein 2; オキシダテブバースト; オキシダティブバースト

The fungal infection specific proteins were detected and identified for the Japanese birch plantlets No.8 infected with a canker-rot fungus of birch trees, Inonotus obliquus IO-U1 strain at the early stage of their fungal infection. As a result, it was found that oxidative burst and systemic acquired resistance occur as the protective reactions in the plantlets No.8 infected with the fungus. In addition, anatomical and histochemical observations were performed for the two kinds of plantlets, No.8 and Tohoku, infected with IO-U1 strain. Consequently, it was suggested that deposition of phenolic compounds and their polymerized substance, and the formation of suberized necrophylactic periderm inhibit the growth of the fungal hyphae in the two kinds of plantlets infected with the fungus. Furthermore, isozyme analysis of the peroxidases produced in the plantlets No.8 was carried out. In consequence, it was indicated that the peroxidase isozymes of the infected plantlets are specialized for polymerization of phenolic compounds. Moreover, it was investigated by using proteome analysis whether systemic acquired resistance (SAR) occurs in the plantlets No.8 to which the SAR-inducing compound, salicylic acid was artificially administered. As a result, it was indicated that administration of salicylic acid to the plantlets No.8 induces SAR in the plantlets. In addition, it was suggested that the occurring resistance mechanisms are different between the case of the fungal infection and that of administration of salicylic acid to the plantlets.

以日本白桦8号试管苗为材料，检测并鉴定了白桦溃疡病菌Inonotus numerus IO-U1菌株侵染初期的侵染特异性蛋白。结果发现，在感染真菌的8号试管苗中，氧化爆发和系统获得抗性作为保护性反应发生。此外，还对感染IO-U1菌株的8号和东北两种试管苗进行了解剖学和组织化学观察。因此，这表明酚类化合物及其聚合物的沉积和木栓化的坏死性菌丝体的形成抑制了真菌菌丝在两种感染真菌的试管苗中的生长。并对8号试管苗的过氧化物酶同工酶进行了分析。因此，这表明，过氧化物酶同工酶的感染苗专门为酚类化合物的聚合。此外，它是通过使用蛋白质组学分析研究是否发生在8号植株的SAR诱导化合物，水杨酸人工管理的系统获得性抗性（SAR）。结果表明，对8号幼苗施用水杨酸诱导了幼苗中的SAR。此外，有人认为，发生的抗性机制之间的情况下，真菌感染和水杨酸的管理到试管苗是不同的。

项目成果

シラカンバNo.8幼植物体におけるサリチル酸応答性タンパク質のプロテオーム解析

8号桦树苗水杨酸响应蛋白的蛋白质组分析

• 发表时间: 2010
• 作者: 鈴木拡;高島有哉;石栗太;飯塚和也;吉澤伸夫;横田信三
• 通讯作者: 横田信三

シラカンバNo.8幼植物体におけるカバノアナタケ菌IO-U1株感染部位縦断面でのフェノール性化合物の堆積及びペルエキシダーゼ活性の経時変化

8 号白桦幼苗真菌 IO-U1 感染部位纵剖面中酚类化合物的沉积和过氧化物酶活性随时间的变化

• 发表时间: 2009
• 作者: 鈴木美帆;高島有哉;石栗太;飯塚和也;吉澤伸夫;横田信三
• 通讯作者: 横田信三

Accumulation of phenolic compounds and activity of peroxidase in Betula platyphylla var. japonica No.8 plantlets infected with Inonotus obliquus IO-U1 strain

白桦酚类化合物的积累及过氧化物酶活性

• 发表时间: 2009
• 作者: M. Suzuki;Y. Takashima;F. Ishiguri;K. Iizuka;N. Yoshizawa;S. Yokota
• 通讯作者: S. Yokota

Defensive histochemical reactions in Japanese birch plantlets infected with Inonotus obliquus IO-U1 strain

感染桦褐孔菌 IO-U1 菌株的日本桦苗的防御性组织化学反应

• 发表时间: 2008
• 作者: M. M. Rahman;M. Suzuki;F. Ishiguri;Y. Takashima;K. Iizuka;N. Yoshizawa;S. Yokota
• 通讯作者: S. Yokota

カバノアナタケ菌IO-U1株に感染したシラカンバ幼植物体No.8における菌感染特異的タンパク出のプロテオーム解析

感染真菌IO-U1的8号白桦幼苗真菌感染特异性蛋白表达的蛋白质组分析

• 发表时间: 2008
• 作者: 高島有哉;M. M. Rahman;石栗太;飯塚和也;吉澤伸夫;横田信三
• 通讯作者: 横田信三