Towards female sterility of Cryptomeria japonica: profile of expression genes at female reproductive organ and analysis of their gene function

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Genetic engineering is a powerful tool for tree breeding with achievement of large genetic gain in short time. However, dispersal of transgenes by pollen or seeds should be prevented. In this study, expression genes in Cryptomeria japonica female reproductive organ was profiled through steps as follows: micro-dissection of micro tissues, ovule, and RNA extraction from the tissue; next generation sequence of ovule- specific suppression subtractive hybridization library; identifications of the ovule specific genes. Our results will be used for female sterilization of no-pollen C. japonica that have been produced by genetic engineering.