Regulatory mechanism of hormone gene expression in the anterior pituitary gland in birds

鸟类垂体前叶激素基因表达的调控机制

• 批准号: 22580322
• 负责人: KANSAKU Norio
• 金额: $ 2.83万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580322/
• 关键词: 転写因子; 下垂体; PREB; PRL; 発生胚; Pit-1; 応用動物; 生体分子

The regulatory mechanism of bird PRL in the anterior pituitary gland is unique and completely different to that of mammalian PRL. The aims of this study were cloning of chicken PREB and comparison of levels of mRNA expression in the various tissues during embryogenesis. Chicken PREB cDNA shows 70% similarity to human and rat PREB cDNAs. The levels of PREB mRNA showed tissue dependent manner. Interestingly, the changes of levels of PREB mRNA had not been observed in the anterior pituitary gland during embryogenesis. These results suggest that PREB protein participate regulation of various gene. Furthermore, PREB involves activation of PRL gene rather than increase of mRNA levels during late stage of embryogenesis.

鸟类PRL在垂体前叶的调节机制是独特的，与哺乳动物PRL的调节机制完全不同。本研究的目的是克隆鸡PREB基因，并比较胚胎发生过程中不同组织中PREB基因的表达水平。鸡PREB cDNA与人和大鼠的PREB cDNA有70%的相似性。PREB mRNA表达水平呈组织依赖性。在胚胎发育过程中，未观察到垂体前叶PREB mRNA水平的变化。提示PREB蛋白参与多种基因的调控。此外，PREB涉及PRL基因的激活，而不是在胚胎发生后期的mRNA水平的增加。

项目成果

Molecular cloning of Prolactin regulatory element binding protein (PREB) in the chicken and turkey

鸡和火鸡催乳素调节元件结合蛋白（PREB）的分子克隆

• 发表时间: 2011
• 作者: Hiyama Gen;Kansaku N;Zadworny D.
• 通讯作者: Zadworny D.

In vitro Suppression of PRL Transcription and Translation in Turkey Embryonic Pituicytes by RNAi.

RNAi 体外抑制火鸡胚胎垂体细胞中 PRL 的转录和翻译。

• 发表时间: 2010
• 作者: G. Hiyama;R. Mcquaid;I.J. Reddy;N. Kansaku;D. Zadworny.
• 通讯作者: D. Zadworny.

Cloning and characterization of prolactin regulatory element binding protein (PREB) in chicken and turkey.

鸡和火鸡催乳素调节元件结合蛋白 (PREB) 的克隆和表征。

• 发表时间: 2012
• 作者: G. Hiyama;N. Kansaku;R.McQuaid;D. Zadworny
• 通讯作者: D. Zadworny

Analysis of cell cycle regulation during quail embryo development. S. Mizushima

鹌鹑胚胎发育过程中细胞周期调控分析。

• 作者: T. Sasanami;N. Kansaku;T. Ono;K. Shimada.
• 通讯作者: K. Shimada.

Role of chicken Pit-1 isoforms in activating growth hormone gene

鸡Pit-1亚型在激活生长激素基因中的作用

• 发表时间: 2011
• 期刊: Gen Comp Endocrinol.
• 作者: Murase D;Taniuchi S;Takeuchi S;Adachi H;Kansaku N;Okazaki K;Ohkubo T.
• 通讯作者: Ohkubo T.