Analysis of function and regulatory mechanism of mRNA expression of bird specific transcription factor in the anterior pituitary gland

鸟类垂体前叶特异性转录因子mRNA表达的功能及调控机制分析

• 批准号: 19580330
• 负责人: KANSAKU Norio
• 金额: $ 3万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580330/
• 关键词: 生産機能制御; 転写制御; Pit-1; アイソフォーム; プロラクチン; TATAbox; レポーターアッセイ; GH3細胞; プロモーター; イントロン; 多型; ヒスタグ

Intron 1 of the precocial and altricial bird of the Pit-1 gene were cloned and sequenced. Homologous position of putative translation starting site of Pit-1w/γ in the altricial species was not ATG methionin codon. Thus, it is suggested that Pit-1w/γ is not exist in the altricial species. Moreover, transcription starting region was cloned by RT-PCR. Results of this study showed that regulatory region of Pit-1w/γ is smaller than previously expected.

克隆了早熟鸟和晚熟鸟Pit-1基因的内含子1并进行了序列测定。Pit-1 w/γ在高等生物中的翻译起始位点不是ATG密码子。因此，推测Pit-1 w/γ在次生种中不存在。此外，通过RT-PCR克隆了转录起始区。本研究结果表明Pit-1 w/γ的调控区域比以前预期的要小。

项目成果

Detection of alternative splicing form of PRL mRNA in the chicken anterior pituitary gland.

鸡垂体前叶PRL mRNA选择性剪接形式的检测。

• 发表时间: 2009
• 作者: N. Kansaku;T. Sasanami;T. Ohkubo;G. Hiyama;D. Zadworny
• 通讯作者: D. Zadworny

Sequence of the domestic duck (Anas platyrhynchos) growth hormone‐encoding gene and genetic variation in the promoter region

• DOI: 10.1111/j.1740-0929.2008.00513.x
• 发表时间: 2008-04
• 期刊: Animal Science Journal
• 影响因子: 2
• 作者: N. Kansaku;A. Soma;S. Furukawa;Gen Hiyama;Hisato Okabayashii;D. Guemene;U. Kuhnlein;D. Zadworny

Sequence comparison of the prolactin (PRL) promoter in BUT and Bettina turkeys

BUT 和 Bettina 火鸡催乳素 (PRL) 启动子的序列比较

• 发表时间: 2008
• 作者: Hiyama;G.;Kansaku;N.;Reddy;I.J.;Sotocinal;S.;Guemene;D.;Zadworny;D.
• 通讯作者: D.

Prolactin and growth hormone in bird: protein structure, gene structure, and genetic variation.

鸟类催乳素和生长激素：蛋白质结构、基因结构和遗传变异。

• 发表时间: 2008
• 期刊: Journal of Poultry Science 45
• 作者: Kansaku;N.;Hiyama;G.;Sasanami;T.;Zadworny.
• 通讯作者: Zadworny.

Comparison of Pit-1 intron-1 in birds.

鸟类中 Pit-1 内含子 1 的比较。

• 发表时间: 2008
• 作者: Kansaku;N.;Tanaka;I.;Tsushima;K.;Zadworny;D.
• 通讯作者: D.