Heterologous expression and large-scale production ofbasidiomycetous lignin-degrading enzyme in heterobasidiomycetousyeast

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It was found that Grifola frondosa produces a laccase (one of lignin-degrading enzymes), which is effective for decolorizing of synthetic dyes and degrading of bisphenol A (recalcitrant environmental pollutant having an endocrine-disrupting effect). The transformation system of heterobasidiomycetous yeast Rhodosporidium toruloides was established using electroporation technique. The attempt of heterologous expression of laccase cDNA in R. toruloides as a host was unsuccessful, however, it was found that Pichia pastoris transformed with the laccase cDNA of G. frondosaproduced a recombinant laccase.