The development of the new RNAi vector system that is effective in analysis and therapy of leukemia and cranial nerve disease.

点击翻译按钮获取中文摘要

In this study, we developed the RNAi vector system which expressed siRNA in cytoplasm using T7 RNA polymerase. The co-transfection of pCMV-T7RNAP and pT7-RNAi-Lu significantly suppressed luciferase expression by pGL3-control plasmid in HeLa cells and A549 cells. Moreover, the co-transfection of pCMV-T7RNAP and pT7-RNAi-p53 significantly suppressed p53 protein expression in HeLa cells. Thus, we were able to confirm that the new vector system using T7 RNA polymerase has function to suppress the expression of target gene.