Understanding of the mechanism underlying the tissue-specific differentiation of high endothelial venule endothelial cells

了解高内皮微静脉内皮细胞组织特异性分化的机制

• 批准号: 22590191
• 负责人: HAYASAKA Haruko
• 金额: $ 2.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590191/
• 关键词: 血管内皮細胞; 細胞分化; リンパ節; 免疫組織; 転写因子; リンパ球

The high endothelial venules (HEVs) are blood vessels specifically found in lymph nodes and Peyer ’ s patches. Although HEVs express specific chemokines/adhesion molecules which mediate lymphocyte t rafficking across the high-walled endothelial cells (HEV -ECs), the mechanism regulating HEVs’ development and maintenance of the unique properties remain unclear. We performed microarray and real -time quantitative PCR analyses of HEV -ECs and non-HEV -ECs inneonatal mice mesenteric LNs, and identified five transcription factors which are over fifty times more abundantly expressed in developing HEV -ECs than in non-HEV -ECs. By immunohistochemical analysis, we found that one of them showed a restricted expression pattern in the nucleus of ECs of a substantial proportion of blood vessels oflymph nodes from E17.5 to the date of birth, which corresponds temporally to HEV -EC development. The gene knockout mice of this transcription factor showed reduced expression of several HEV -associated genes, implying the functionalcontribution of this gene to HEV -EC differentiation.

高内皮微静脉（HEV）是在淋巴结和派尔集合淋巴结中特异性发现的血管。尽管HEV表达介导淋巴细胞穿过高壁内皮细胞（HEV-EC）的特异性趋化因子/粘附分子，但调节HEV的发展和维持独特性质的机制仍不清楚。我们对新生小鼠肠系膜淋巴结中HEV-EC和non-HEV -EC进行了微阵列和真实的实时定量PCR分析，并鉴定了五种转录因子，它们在发育中的HEV-EC中的表达量是non-HEV -EC的50倍以上。通过免疫组化分析，我们发现其中一个在E17.5至出生日期间在相当大比例的淋巴结血管内皮细胞核中显示限制性表达模式，这在时间上对应于HEV-EC的发展。该转录因子基因敲除小鼠表现出几个HEV相关基因的表达降低，暗示该基因对HEV-EC分化的功能贡献。

项目成果

The effects of HIV-1 gp120 on CCR7 ligand-induced human CD4 T cell migration

HIV-1 gp120对CCR7配体诱导的人CD4 T细胞迁移的影响

• 发表时间: 2010
• 作者: Hayasaka H.;Yoshimura H.;Nakayama E.E.;Shioda T.;Bai Z.;Miyasaka M
• 通讯作者: Miyasaka M

The effect of HIV-1 gp120 on CCR7 ligand-induced human CD4 T cell trafficking

HIV-1 gp120 对 CCR7 配体诱导的人 CD4 T 细胞运输的影响

• 发表时间: 2010
• 作者: Denda-Nagai K;Aida S;Saba K;Suzuki K;Moriyama S;Oo-Puthinan S;Tsuiji M;Morikawa A;Kumamoto Y;Sugiura D;Kudo A;Akimoto Y;Kawakami H;Bovin NV;Irimura T.;Hayasaka H
• 通讯作者: Hayasaka H

he HIV-1 gp120/CXCR4 interaction promotes CCR7-dependent CD4 T cell trafficking into lymph nodes.

HIV-1 gp120/CXCR4 相互作用促进 CCR7 依赖性 CD4 T 细胞转运至淋巴结。

• 发表时间: 2012
• 作者: Hayasaka H.;Yoshimura H.;Kobayashi D.;Nakayama E.E.;Shioda T.;Bai Z.;Miyasaka M.
• 通讯作者: Miyasaka M.

Constitutive Lymphocyte Transmigration across the Basal Lamina of High Endothelial Venules Is Regulated by the Autotaxin/Lysophosphatidic Acid Axis

• DOI: 10.4049/jimmunol.1202025
• 发表时间: 2013-03-01
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Bai, Zhongbin;Cai, Linjun;Miyasaka, Masayuki
• 通讯作者: Miyasaka, Masayuki

Two-State Conformations in the Hyaluronan-Binding Domain Regulate CD44 Adhesiveness under Flow Condition

• DOI: 10.1016/j.str.2010.02.010
• 发表时间: 2010-05-12
• 期刊: STRUCTURE
• 影响因子: 5.7
• 作者: Ogino, Shinji;Nishida, Noritaka;Shimada, Ichio
• 通讯作者: Shimada, Ichio