The source of acetylcholine acting to the renal tubules.

为阐明作用于肾脏的乙酰胆碱（ACh）的来源，用神经元示踪法和组织化学方法分别观察了大鼠肾脏副交感神经支配和非神经元胆碱乙酰转移酶（ChAT）的表达。逆行神经元示踪剂Fluoro-gold通过微囊处理肾神经末端，结果在延髓和脊髓的任何副交感神经核中没有阳性细胞。另一方面，乙酰胆碱合成酶ChAT定位于肾皮质集合管，在肾小管中分布不均匀。这些结果表明，在大鼠肾脏中，非神经性ACh可能比神经性ACh占优势。

To clarify the source of acetylcholine (ACh) which acts to the kidney, parasympathetic innervations and non-neuronal expression of choline acetyl transferase (ChAT) were examined in the rat kidney by using a neuronal tracing method and histochemical methods, respectively. Retrograde neuronal tracer Fluoro-gold was treated to the renal nerve ends by a micro-capsule, resulting that there were no positive cells in any parasympathetic nuclei in the medulla oblongata and spinal cords. On the other hand, ACh-synthesizing enzyme ChAT was localized to the renal cortical collecting ducts with uneven distributions in the tubules. These results suggest that the non-neuronal ACh may be predominant over the neuronal ACh in the rat kidney.