Functional role of the transient receptor potential canonical (TRPC) channels in the development of cardiac ischemia/reperfusion injury

瞬时受体电位经典（TRPC）通道在心脏缺血/再灌注损伤发展中的功能作用

• 批准号: 22590205
• 负责人: MATSUURA Hiroshi
• 金额: $ 2.83万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590205/
• 关键词: 生体膜; チャネル; トランスポーター; 能動輸送; 心筋虚血再灌流傷害; TRPCチャネル; Ca2+過負荷; 細胞過拘縮; ランゲンドルフ灌流法; 2-APB; LaCl3; 再疎通療法; 活性酸素種; 過酸化水素; 共焦点レーザ顕微鏡; 細胞内Ca^<2+>イメージング; 過拘縮; パッチクランプ法; Ca^<2+>パラドックス; マウス心室筋細胞; fluo-3; 虚血再灌流傷害; Ca^<2+>過負荷

Ca2+overload has been implicated in the pathogenesis of ischemia/reperfusion injury in the heart. The present investigation used Langendorff perfusion method of mouse hearts and fluorescence Ca2+imaging of mouse ventricular myocytes and revealed that the transient receptor potential canonical (TRPC) channels mediate the Ca2+entry responsible for Ca2+overload during the reperfusion of ischemic myocardium. These observations indicate that TRPC channel blockade could provide potential new avenues to develop strategies for protecting the heart during reperfusion of ischemic myocardium.

钙超载参与了心脏缺血/再灌注损伤的发病机制。本研究采用小鼠心脏的Langendorff灌流法和小鼠心肌细胞的荧光钙显像法，发现在缺血心肌再灌流过程中，瞬时受体电位通道(TRPC)介导了导致钙超载的钙内流。这些观察表明，TRPC通道阻断可能为开发缺血心肌再灌注期间的心脏保护策略提供潜在的新途径。

项目成果

Sevoflurane protects ventricular myocytes from Ca2+paradox-mediated Ca2+overload by blocking the activation of transient receptor potential canonical channels

七氟烷通过阻断瞬时受体电位经典通道的激活，保护心室肌细胞免受 Ca2 悖论介导的 Ca2 超载

• DOI: 10.1097/aln.0b013e31822b7901
• 发表时间: 2011
• 期刊: Anesthesiology
• 影响因子: 8.8
• 作者: Kojima A;Kitagawa H;Omatsu-Kanbe M,Matsuura H;Nosaka S
• 通讯作者: Nosaka S

Nosaka S. Sevoflurane protects cardiomyocyte from Ca2+paradox by preserving SR Ca2+and blocking TRPC channel

Nosaka S.七氟烷通过保留 SR Ca2 并阻断 TRPC 通道来保护心肌细胞免受 Ca2 悖论

• 发表时间: 2010
• 作者: Kojima A;Kitagawa H;Omatsu-Kanbe M;Matsuura H
• 通讯作者: Matsuura H

Ca2+ paradox injury mediated through TRPC channels in mouse ventricular myocytes

• DOI: 10.1111/j.1476-5381.2010.00986.x
• 发表时间: 2010-12-01
• 期刊: BRITISH JOURNAL OF PHARMACOLOGY
• 影响因子: 7.3
• 作者: Kojima, Akiko;Kitagawa, Hirotoshi;Nosaka, Shuichi
• 通讯作者: Nosaka, Shuichi

The improved effect of hypothermic cultivation on the functional expression of human cardiac Kv1.5 channels

低温培养对人心脏Kv1.5通道功能表达的改善作用

• 发表时间: 2013
• 作者: Ding W-G;Xie Y;Toyoda F;Bai JY. Matsuura H
• 通讯作者: Bai JY. Matsuura H

Ischemic survival and constitutively active autophagy in self-beating atypically-shaped cardiomyocytes (ACMs): characterization of a new subpopulation of heart cells

• DOI: 10.1007/s12576-012-0236-5
• 发表时间: 2012-10
• 期刊: The Journal of Physiological Sciences
• 作者: M. Omatsu-Kanbe;H. Matsuura