Molecular mechanisms of the regulation of oligosaccharides within egg-coating envelope during fertilization

受精过程中卵包膜内寡糖调控的分子机制

• 批准号: 22590213
• 负责人: MIWA Naofumi
• 金额: $ 2.5万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590213/
• 关键词: 生理学; 受精; 糖鎖; 発生・分化; 糖類; 糖蛋白質

Fertilization comprises oligosaccharide-mediated sperm-egg interactions, beginning with sperm-recognition of egg-coating extracellular envelope. We identified a novel mediator of fertilization, dicalcin, in the egg envelope of Xenopus laevis. Inactivation of native dicalcin by specific antibody increased the fertilization rate, while addition of dicalcin inhibits fertilization as well as sperm-recognition. Dicalcin binds to a constitutive glycoprotein of the egg envelope, inducing its conformational change, and simultaneously affects the structure of the envelope. We also identified the mouse counterpart of Xenopus dicalcin, and found thatmouse dicalcin, present in the cumulus cells of cumulus-oocyte complex, also inhibits in vitro fertilization. Thus, dicalcin regulates sperm-egg interaction during fertilization both in frogs and mice

受精包括寡糖介导的精子与卵子的相互作用，首先是精子识别卵子包被的细胞外包膜。我们在非洲爪哇的卵膜中发现了一种新的受精介体--双钙素。用特异性抗体灭活天然大豆素可以提高受精率，而加入大豆素则会抑制受精和精子识别。Dicalcin与卵膜的一种构成糖蛋白结合，引起其构象变化，同时影响卵膜的结构。我们还鉴定了非洲爪哇的小鼠对应物，并发现存在于卵丘-卵母细胞复合体的卵丘细胞中的小鼠双钙素也抑制体外受精。因此，在青蛙和小鼠受精过程中，双钙素能调节精子与卵子的相互作用。

项目成果

Aging of Egg-Coating Structures and Dicalcin

蛋壳结构和双钙素的老化

• 发表时间: 2011
• 期刊: J Mamm Ova Res
• 作者: Hanaue M.;et al
• 通讯作者: et al

Dicalcin Inhibits Fertilization through Its Binding to a Glycoprotein in the Egg Envelope in Xenopus laevis

• DOI: 10.1074/jbc.m109.079483
• 发表时间: 2010-05-14
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Miwa, Naofumi;Ogawa, Motoyuki;Kawamura, Satoru
• 通讯作者: Kawamura, Satoru

Characterization of Mouse Dicalcin in the Female Reproductive Tissue

小鼠双钙素在雌性生殖组织中的表征

• 发表时间: 2012
• 作者: 三輪尚史;花上まゆ;高松研
• 通讯作者: 高松研

Characterization of the mouse counterpart of frog dicalcin in the female reproductive tissue

雌性生殖组织中青蛙双钙蛋白的小鼠对应物的表征

• 发表时间: 2012
• 作者: 三輪尚史;花上まゆ;高松研
• 通讯作者: 高松研

Characterization of Mouse Dicalcin, a Potential Suppressive Factor of Fertilization, in the Mouse Female Reproductive Tract

小鼠双钙素（一种潜在的受精抑制因子）在小鼠雌性生殖道中的表征

• 发表时间: 2011
• 作者: Miwa N;Hanaue M;Takamatsu K
• 通讯作者: Takamatsu K