权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Functional analysis of olfactory Eik/MAPK in olfactory plasticity

嗅觉Eik/MAPK在嗅觉可塑性中的功能分析

基本信息

批准号：
18500249
负责人：
MIWA Naofumi
金额：
$ 2.6万
依托单位：
Toho University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500249/
关键词：
olfaction Erk MAPK

项目摘要

We examined whether continuous odorant stimuli would affect olfactory-related behavior as a final output of olfactory processing. By our behavioral assay, we analyzed odorant dose -dependency after isoamyl-acetate (IAA) was continuously delivered to mice for several weeks. We have found that continuous odorant delivery augments olfactory sensitivity to IAA up to 10 times. Our preferential test using IAA and other odorant have shown that these mice have a preference to IAA. To elucidate the mechanism of this olfactory sensitization, we examined the effect of Erk/MAPK, a principal kinase in neuron, on this behavioral augmentation. Administration of specific inhibitor of Erk/MAPK abolished this augmentation, reducing the sensitivity to normal level. We, next, examined olfactory sensitization at the level of olfactory epithelium. Brief administration of IAA transiently activated Erk/MAPK in olfactory neurons. Continuous administration of IAA increased the number of BrdU-immunopositive olfactory neurons. These results indicated that continuous delivery of odorant promotes survival of olfactory neurons through undefined mechanisms possibly mediating Erk/MAPK activation. This could underlie olfactory sensitization at the level of olfactory epithelium. We are examining molecular events in olfactory neurons under this condition to elucidate the mechanism of survival of olfactory neurons.

我们研究了连续的气味刺激是否会影响嗅觉相关的行为作为嗅觉加工的最终输出。通过我们的行为测定，我们分析了乙酸异戊酯（IAA）连续递送给小鼠数周后的气味剂量依赖性。我们已经发现，连续的气味传递增加嗅觉灵敏度IAA高达10倍。我们使用IAA和其他气味剂的偏好性测试表明，这些小鼠对IAA具有偏好性。为了阐明这种嗅觉敏化的机制，我们研究了Erk/MAPK，神经元中的主要激酶，对这种行为增强的影响。给予Erk/MAPK特异性抑制剂可消除这种增强作用，使敏感性降至正常水平。接下来，我们在嗅上皮的水平上检查嗅觉敏感性。短暂给予IAA瞬时激活嗅觉神经元中的Erk/MAPK。连续给予IAA增加BrdU免疫阳性嗅神经元的数量。这些结果表明，连续递送气味剂通过可能介导Erk/MAPK激活的未定义的机制促进嗅觉神经元的存活。这可能是在嗅上皮水平上嗅觉敏感的基础。我们正在研究在这种情况下嗅觉神经元的分子事件，以阐明嗅觉神经元的生存机制。