Functional analyses on the glycosylation in cardiac calcium-regulating proteins and ion channels

心脏钙调节蛋白和离子通道糖基化的功能分析

• 批准号: 22590297
• 负责人: ASAHI Michio
• 金额: $ 2.91万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590297/
• 关键词: 分子病態学; 糖鎖; 循環器・高血圧; 糖尿病

The glycosylation is one of the major post-translational modification, which regulates the function of various proteins. There are two types of glycosylation, N-linked and O-linked. Here, I studied about the effect of O-GlcNAcylation, one of the O-linked glycosylation, on the function of cardiac calcium-regulating proteins and ion channels. Although we examined such various proteins in rat adult cardiomyocytes, it was found that O-GlcNAcylation of phospholamban, which plays a crucial role in the regulation of cardiac function, was increased, and, simultaneously, its phosphorylation was inhibited, resulting in the reduction of cardiac function.

糖基化是主要的翻译后修饰之一，调节着多种蛋白质的功能。糖基化有两种类型，N-连接和O-连接。在这里，我研究了O-连接糖基化之一的O-GlcN酰化对心肌钙调节蛋白和离子通道功能的影响。虽然我们在成年大鼠心肌细胞中检测了这些不同的蛋白质，但发现在心功能调节中起关键作用的磷蛋白的O-GlcN酰化增加，同时其磷酸化被抑制，导致心功能下降。

项目成果

ラット小腸上皮細胞IEC-6 に おける lansoprazole による HO-1 発現誘導分子機構の検討

兰索拉唑诱导大鼠小肠上皮细胞IEC-6表达HO-1的分子机制研究

• 发表时间: 2012
• 作者: 依田有紀子;中川孝俊、 朝日通雄
• 通讯作者: 中川孝俊、 朝日通雄

Preparation, characterization, and biological evaluation of 6^I, 6^<IV>-di-O-[α-L-fucopyranosyl-(1→6)-2-acetamido-2-deoxy-β-D-glucopyranosyl]-cyclomaltoheptaose and 6-O-[α-L-fucopyranosyl-(1→6)-2-acetamido-2-cleoxy-β-D-glucopyranosyl]-cyclomaltoheptaose

6^I, 6^<IV>-di-O-[α-L-吡喃岩藻糖基-(1→6)-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖基]-的制备、表征和生物学评价环麦芽七糖和 6-O-[α-L-吡喃岩藻糖基-(1→6)-2-乙酰氨基-2-环氧基-β-D-吡喃葡萄糖基]-环麦芽七糖

• 发表时间: 2011
• 期刊: Carbohydrate Research
• 影响因子: 3.1
• 作者: 山本隼也;萩谷至史;鎌田祥太郎;笠原忠;石井功;永井尚子;安藝翔;Nakagawa T
• 通讯作者: Nakagawa T

N-glycans of SREC-I (scavenger receptor expressed by endothelial cells): essential role for ligand binding, trafficking and stability.

• DOI: 10.1093/glycob/cws010
• 发表时间: 2012-05
• 期刊: Glycobiology
• 影响因子: 4.3
• 作者: M. Sano;H. Korekane;K. Ohtsubo;Y. Yamaguchi;Masaki Kato;Yukinao Shibukawa;M. Tajiri;H. Adachi;Y. Wada;M. Asahi;N. Taniguchi

Cardiac adipose tissue-derived stem cells exhibit high differentiation potential to cardiovascular lineage cells

心脏脂肪组织来源的干细胞对心血管谱系细胞表现出高分化潜力

• 发表时间: 2011
• 作者: 坂下英司;遠藤仁司;Hiroki Nagata
• 通讯作者: Hiroki Nagata

ラット小腸上皮細胞IEC-6におけるlansoprazole によるHO-1発現誘導分子機構の検討

兰索拉唑诱导大鼠小肠上皮细胞IEC-6表达HO-1的分子机制研究

• 作者: Tamemoto;H.;Ozaki;H.;Kamoshita;N.;Hayakawa;M.;and Tominaga;S.;依田有紀子
• 通讯作者: 依田有紀子