Functional analyses on the glycosylation in cardiac calcium-regulating proteins and ion channels

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The glycosylation is one of the major post-translational modification, which regulates the function of various proteins. There are two types of glycosylation, N-linked and O-linked. Here, I studied about the effect of O-GlcNAcylation, one of the O-linked glycosylation, on the function of cardiac calcium-regulating proteins and ion channels. Although we examined such various proteins in rat adult cardiomyocytes, it was found that O-GlcNAcylation of phospholamban, which plays a crucial role in the regulation of cardiac function, was increased, and, simultaneously, its phosphorylation was inhibited, resulting in the reduction of cardiac function.