Development of a glomerulonephritis therapeutic modelwith novel protein analogs targeting the polymorphic binding site of osteopontin (Opn)

我们提出了一种新的蛋白类似物抑制骨桥蛋白（Opn）结合位点的治疗模型，骨桥蛋白是肾小球肾炎易感性多态性基因产物之一。通过α筛选系统和gst捕获ELISA对Opn功能的抑制蛋白进行筛选，然后进行体内和体外实验，结果抑制t细胞活化和肾小球肾炎的发展。

We objected to propose a therapeutic model with a certain kind of novel protein analogs inhibiting the binding site of osteopontin (Opn) which is one of the susceptibility polymorphic gene product to glomerulonephritis in mouse model. Screening of the inhibiting protein against Opn function was carried out by Alpha Screen system and GST-capture ELISA followed by the in vivo andin vitro assays which resulted in the suppression of T-cell activation as well as thedevelopment of glomerulonephritis.