Examination of rapid and easy detection methods for fungal beta-glucans

为了建立真菌产物，特别是真菌患者血液中β -葡聚糖的特异性检测方法，我们检测了来自无脊椎动物和哺乳动物的各种β -葡聚糖识别蛋白（BGRP）。通过比较重组BGRPs对不同天然来源（真菌、细菌、藻类和植物）β -葡聚糖的结合特异性，选择合适的蛋白探针进行检测。将所选BGRPs联合应用ELISA法和免疫层析法。利用这些设备，我们可以检测真菌感染动物模型血液中痕量的β -葡聚糖。

To develop a specific detection methods for fungal products, particularly beta-glucans found in blood of mycosis patient, we examined various beta-glucan recognition proteins (BGRP) from invertebrates and mammalian. The binding specificities of the recombinant BGRPs to the beta-glucans from various natural source, including fungi, bacterium, algae, and plants were compared to choose suitable protein probes for the detection. The combination of selected BGRPs was applied for ELISA methods and immunochromatography. Using those devices, we could detect trace level of beta-glucans in the blood from animal model of fungal infection.