Development andapplication of the new clinical laboratory procedure using saliva at home medical care-For the prevention of senile pneumonia-

家庭医疗唾液临床检验新流程的开发与应用-预防老年肺炎-

• 批准号: 22590548
• 负责人: SHIRAKAWA Taku
• 金额: $ 2.91万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590548/
• 关键词: 老人性肺炎; 抗菌ペプチド; β-ディフェンシン(hBD); ELISA; C-反応性蛋白(CRP); 唾液; 炎症マーカー; β－ディフェンシン（ｈBD); C-反応性タンパク（CRP）; β-ディフェンシン2(hBD2); C反応性タンパク(CRP); 唾液採取法; イムノクロマトグラフィー; 可溶性Toll様受容体(sTLR)

Both CRP and human beta defensin(hBD) concentration in saliva were used for the early detection and prevention of senile pneumonia. CRP was recognized as an inflammatory marker of pneumonia and hBD, as an index of thecleanliness level in oral cavity, respectively. We found a good association between CRP measured in saliva and serum of an inflammatory disorder patients (r=0.88,P < 0.001). Furthermore, when the transferrin concentration in saliva was limited to a less than 1 mg/ml sample, the association was become stronger (r=0.96,P < 0.001). Moreover, some pretreating methods of saliva were examined for the measurement of hBD with positive charge. Tween 20 and diluted hydrochloric acid were used to release hBD from negative charged matter, such as muchin. Measurement of the exact hBD concentration in salivawas attained by this pretreating method. These results suggested that saliva test would be useful for the early detection and prevention of senile pneumonia.

唾液中CRP和人β防御素（hBD）浓度的测定可用于老年性肺炎的早期检测和预防。CRP是肺炎的炎症标志物，hBD是口腔清洁度的指标。炎症性疾病患者唾液CRP与血清CRP之间存在良好的相关性（r= 0.88，P < 0.001）。当唾液中转铁蛋白浓度小于1 mg/ml时，唾液中转铁蛋白浓度与唾液中转铁蛋白浓度的相关性更强（r= 0.96，P < 0.001）。此外，还对唾液的几种前处理方法进行了研究，以用于带正电荷的hBD的测定。用吐温20和稀盐酸从带负电荷的物质如muchin中释放hBD。用此方法可准确测定唾液中hBD的浓度。提示唾液检测对老年肺炎的早期发现和预防有重要意义。

项目成果

唾液中CRP高感度測定法開発のための基礎的検討

开发高灵敏度唾液CRP测定方法的基础研究

• 发表时间: 2011
• 作者: 成田祐美、白川卓;他
• 通讯作者: 他

唾液中 CRP 高感度測定法開発のための基礎的検討

开发唾液中CRP高灵敏度测定方法的基础研究

• 发表时间: 2011
• 作者: 成田祐美;白川 卓他
• 通讯作者: 白川 卓他

Development of high-sensitivity assay for C-reactive protein in human saliva

开发人类唾液中 C 反应蛋白的高灵敏度检测方法

• 发表时间: 2012
• 作者: Yumi Narita;Taku Shirakawa
• 通讯作者: Taku Shirakawa