Mechanism analyses of development of cardiovascular lesions in long-time workers by multi-biomarkers.

长时间工作会增加心血管疾病，即使是年轻工人。然而，其机制尚未阐明。我们分析了生物标志物，以阐明日本办公室工作人员长时间工作诱发心血管疾病发展的病理生物化学机制。每月工作超过240小时的上班族（每月加班> 80小时），我们检测了以下指标：白细胞介素-6;基质金属蛋白酶-9;肿瘤坏死因子-α;脂联素;小而密低密度脂蛋白（LDL）;丙二醛修饰的低密度脂蛋白（MDA-LDL）;总胆固醇; LDL胆固醇;高密度脂蛋白胆固醇;同型半胱氨酸;正五聚蛋白3;和高敏C反应蛋白77.4%的工人有异常高的MDA-LDL。当他们的工作时间少于每月240小时时，再次测量相同的标记。与每月工作时间> 240小时相比，工作时间较短的人，MDA-LDL与LDL的比率显着降低。其他生物标志物在较长和较短的工作时间之间没有显著变化。这些发现表明，长时间工作可能会加速动脉粥样硬化病变的形成。监测MDA-LDL与总LDL的比值可能为长时间工作的工人的健康提供有用的信息。降低氧化型低密度脂蛋白可能对长时间工作的上班族预防心血管事件具有重要意义。

Working long hours increases cardiovascular diseases even in young workers. However, the mechanisms for this have not yet been elucidated. We analyzed biomarkers to clarify the pathobiochemical mechanisms involved in the development of cardiovascular diseases induced by working long hours in Japanese office workers. In office workers who worked over 240 hours a month (> 80 hours of overtime work a month), we measured the following: interleukin-6; matrix metalloproteinase-9; tumor necrosis factor-α; adiponectin; small dense low-density lipoprotein (LDL); malondialdehyde-modified LDL (MDA-LDL); total cholesterol; LDL cholesterol; high-density lipoprotein cholesterol; homocysteine; pentraxin 3; and high-sensitivity C-reactive protein. Of the workers, 77.4% had abnormally high MDA-LDL. The same markers were measured again when their working hours were shorter than 240 hours a month. With shorter working hours compared to working hours > 240 hours a month, the ratio of MDA-LDL to LDL significantly decreased. There were no significant changes in other biomarkers between the longer and shorter working hours. These findings suggest that working long hours might accelerate the formation of atherosclerotic lesions. Monitoring the ratio of MDA-LDL to total LDL might provide useful information for the healthcare of workers with long working hours. Lowering oxidized LDL might be of importance and beneficial in preventing cardiovascular events in office workers with long working hours.