Comprehensive analysis of micro RNA using mass-spectrometry and its forensic application

点击翻译按钮获取中文摘要

Non-coding and small single-stranded RNA molecules, microRNAs (miRNAs), have been found to regulate a variety of physiological functions. We focused on the behavior of miRNAs at postmortem as a biomarker to quantify the physical stress. In this preliminary study on rat, we determined a number of miRNAs that exist after death and found their possibility as a useful tool to as a biomarker to determine the cause of death. Unfortunately, this study revealed that it was prohibitively difficult to analyze the total RNA including miRNAs using the LC/MS/MS directly. We performed comprehensive microarray analysis of rat miRNAs after their death.