Simultaneous sensitive detection of chelated medicines and poisonsby electrospray ionization tandem mass spectrometry without column separation

电喷雾电离串联质谱无需柱分离同时灵敏检测螯合药物和毒物

• 批准号: 22590631
• 负责人: MINAKATA Kayoko
• 金额: $ 2.91万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590631/
• 关键词: 薬毒物; 錯体; タンデム質量分析; イオンスプレー; 質量分析; 過塩素酸; ヨード; 金; 銅; アジ化ナトリウム; Au; 錯体化合物; シアン; 胃内容; 尿; 血液; 練炭中毒

To facilitate the detection of highly hydrophilic medicines and poisons, we developed a method that produces chelate complexes of analytes and then extracts them with polar organic solvents before electrospray ionization mass spectrometric (ESI-MS) detection. During the study we noticed the possibility to detect several analytes simultaneously by the method. In the simultaneous detection, usually, column separation was performed to separate analytes as well as to eliminate impurities. In tandem mass spectrometry (MS -MS), MS detection was performed twice in a blink for precursor ion and product ion, and hence impurities were eliminated twice. Most analytes having different molecular structures showed different product ions at the 2ndMS even though they showed the same m/z at the 1stMS. Therefore, our simultaneous detection method by ESI-MS-MS did not requirecolumn separation that took about 30 min. Using this method, three groups of analytes, that is, 1. cyanide-azide ion group, 2. coinage-metal group, 3. ion group that related to hypothyroidism, could be detected simultaneously during these three years. Multi analytes detection using a single sample without column separation contributes greatly to the saving of time and resources in forensic and clinical toxicology.

为了方便高亲水性药物和毒物的检测，我们开发了一种方法，在电喷雾电离质谱（ESI-MS）检测之前，将分析物生成螯合物，然后用极性有机溶剂提取它们。在研究过程中，我们注意到该方法可以同时检测几种分析物。在同时检测中，通常进行柱分离以分离分析物并去除杂质。串联质谱法（MS -MS）在一瞬间对前体离子和产物离子进行两次质谱检测，从而消除了两次杂质。大多数具有不同分子结构的分析物，虽然在第一次质谱分析中显示出相同的m/z，但在第二次质谱分析中显示出不同的产物离子。因此，我们的ESI-MS-MS同时检测方法不需要大约30分钟的柱分离。使用这种方法，三组分析物，即1。叠氮化氰离子基，2。铸币金属组，3。与甲状腺功能减退有关的离子群，在这三年中可以同时检测到。使用单一样品进行多分析物检测，无需柱分离，大大节省了法医和临床毒理学的时间和资源。

项目成果

柔道練習中の死亡事故1例

柔道练习过程中发生死亡事故1起

• 发表时间: 2011
• 作者: Ibuki C;Seino Y;Mizuo K;山岸格
• 通讯作者: 山岸格

Determination of azide in gastric fluid and urine by flow-injection electrospray ionization tandem mass spectrometry

流动注射电喷雾电离串联质谱法测定胃液和尿液中的叠氮化物

• DOI: 10.1007/s00216-012-5848-3
• 发表时间: 2012
• 期刊: Analytical Bioanalytical Chemistry
• 作者: Kayoko Minakata;Hideki Nozawa
• 通讯作者: Hideki Nozawa

Simultaneous analysis of cardiac glycosides in blood and urine by thermoresponsive LC-MS-MS

热响应 LC-MS-MS 同时分析血液和尿液中的强心苷

• DOI: 10.1007/s00216-010-4405-1
• 发表时间: 2011
• 期刊: Analytical Bioanalytical Chemistry
• 作者: Sanae Kanno;Kanako Watanabe;Itaru Yamagishi;Seishiro Hirano;Kayoko Minakata;Kunio Gonmori;Osamu Suzuki
• 通讯作者: Osamu Suzuki

A sensitive and fully automated method for GC-MS analysis of volatile organic compounds in whole blood using large volumes of headspace vapor.

一种灵敏且全自动的方法，使用大量顶空蒸汽对全血中的挥发性有机化合物进行 GC-MS 分析。

• 发表时间: 2012
• 作者: Koutaro Hasegawa;Amin Wurita;Kunio Gonmori;Hideki Nozawa;Itaru Yamagishi;Kayoko Minakata;Osamu Suzuki;Kanako Watanabe
• 通讯作者: Kanako Watanabe

胃内にPTP(press through package)包装の一部と推定されるアルミ片を認めた3例

在三起案件的胃中发现了据信是 PTP（压穿包装）包装一部分的铝片。

• 发表时间: 2012
• 作者: 山岸格;野澤秀樹;長谷川弘太郎;権守邦夫;南方かよ子;鈴木修;渡部加奈子
• 通讯作者: 渡部加奈子