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Elusidation of the enhancing factor in paraquat toxicity under magnesium restriction

镁限制下百草枯毒性增强因素的阐明

基本信息

批准号：
10670385
负责人：
MINAKATA Kayoko
金额：
$ 2.24万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670385/
关键词：
paraquat magnesium copper iron zinc calcium reactive oxygen atomic absorption spectrometry マンガン ESR ラジカルジクワット活性酵素抗酸化剤ビタミンC 電子スピン共鳴

项目摘要

A highly sensitive but simple method for the quantification of Cu was devised (published in Clin Chem, 2001, JpnJ Forensic Toxicol, 2001). Only 5μl of plasma or 1.5 ml of running water is enough to quantify Cu. By use of this method tissue Cu levels of rats intoxicated with paraquat at 250 ppm in the diet were examined. The contents of Fe, Mg and Zn were determined by atomic absorption spectrometry, Shimadsu AA-6200 which was obtained by the present Grant-in-Aid. In intoxicated rats, Cu increased 2-fold of the control in lung, liver or plasma, and decreased to 0.5-foldin kidney. Fe increased 2-fold in liver or spleen, and decreased to 0.5-fold in plasma. Mg increased 2-fold in kidney. Liver Mg or Zn increased significantly (J Toxicol Environ Health, 2002)Mg restricted rats dosed with 125 ppm paraquat gave the same changes in these minerals (JpnJ Legal Med, 2001). However, Ca in kidney increased 10-foldin Mg restricted rats (will be reportedinThe86th Congress ofJpn Soc Legal Med, 2002). Usually it takes more than 35days to accumulate Ca at such a high level by dietary Mg restriction only. Feeding paraquat at 125 ppm under Mg restriction accelerated both paraquat toxicosis and Mg deficiency. The kidney Ca of rats fed with 125 ppm paraquat under all mineral restriction increased to 20-fold, where the ratio of Ca to Mg in the diet was maintained (will be reported in ISALM, 2002).

设计了一种高灵敏度但简单的铜定量方法（发表于clinin Chem, 2001; JpnJ Forensic Toxicol, 2001）。仅5μl血浆或1.5 ml自来水就足以定量铜。用该方法测定了百草枯中毒大鼠250 ppm的组织铜水平。铁、镁、锌的含量用本次资助项目获得的岛津AA-6200原子吸收光谱法测定。中毒大鼠肺、肝、血浆中Cu含量均较对照组增加2倍，肾脏中Cu含量降至0.5倍。铁在肝脏和脾脏中升高2倍，血浆中降低0.5倍。Mg在肾脏中增加2倍。肝脏中Mg或Zn显著增加（《毒物与环境健康杂志》，2002年）。剂量为125 ppm百草枯的Mg限制大鼠在这些矿物质中也出现了同样的变化（《日本法律医学杂志》，2001年）。然而，限镁大鼠肾脏钙含量增加了10倍（将在第86届日本医学会法律医学大会上报道，2002年）。一般情况下，仅限镁需要35天以上才能积累如此高水平的钙。在限制Mg含量的情况下，饲喂125 ppm的百草枯加速了百草枯中毒和Mg缺乏症。在所有矿物质限制条件下，喂食125 ppm百草枯的大鼠的肾脏钙增加到20倍，而饮食中钙与镁的比例保持不变（将在ISALM， 2002年报道）。