Elucidation of the mechanisms involved in development and suppression ofBarrett's esophagus: Results of joint Japan-U.S. research

阐明巴雷特食管的发育和抑制机制：日美联合研究的结果

• 批准号: 22590692
• 负责人: WATARI Jiro
• 金额: $ 2.75万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590692/
• 关键词: 上部消化管学(食道,胃,十二指腸); バレット食道; 特殊腸上皮化生; 円柱上皮化生; プロトンポンプ阻害薬; メチル化異常; マイクロサテライト不安定性; Das-1抗体; ゲノム不安定性; Helicobacter pylori

We evaluated several molecular alterations including microsatellite instability (MSI); the CpG island methylation status of hMLH1, p16, E-cadherin and APC genes; and monoclonal antibody Das-1, which specifically reacts with BE in specialized intestinal metaplasia (SIM), as well as in columnar lined epithelium (CLE) without SIM in BE mucosa. Additionally, we investigated changes in the above molecular markers in a randomized control trial, comparing PPI administration versus PPI non-administration groups. Our results showed no significant differences in the frequency of MSI, or methylation at any genes investigated in SIM or CLE. In contrast, Das-1 reactivity was significantly higher in SIM versus CLE. The frequency of methylation and Das-1 reactivity among Japanese was similar to that of BE in the U.S. population, a finding which corroborates previous reports. In patients administered PPI, MSI and hypermethylation at p16, E-cadherin, and APC genes disappeared following interventon, while patients without PPI did not show this tendency. These results indicate that CLE may be a precancerous lesion as well as SIM, and there is no difference in the molecular characteristics of BE between Japanese and U.S. populations. Furthermore, PPI may promote regression of the molecular alterations involved in BE.

我们评估了几种分子改变，包括微卫星不稳定性（MSI）；hMLH1、p16、E-cadherin和APC基因的CpG岛甲基化状态；单克隆抗体Das-1，在特化肠化生（SIM）和BE粘膜无SIM的柱状衬上皮（CLE）中与BE特异性反应。此外，我们在一项随机对照试验中研究了上述分子标记物的变化，比较PPI给药组和未给药组。我们的研究结果显示，在SIM或CLE研究的任何基因的MSI或甲基化频率上没有显着差异。相反，与CLE相比，SIM中的Das-1反应性明显更高。日本人的甲基化频率和Das-1反应性与美国人的BE相似，这一发现证实了之前的报道。在使用PPI的患者中，MSI和p16、E-cadherin和APC基因的高甲基化在干预后消失，而未使用PPI的患者则没有这种趋势。这些结果表明CLE可能是一种癌前病变和SIM，并且在日本和美国人群中be的分子特征没有差异。此外，PPI可能促进BE中涉及的分子改变的消退。

项目成果

肥満と消化器疾患

肥胖和消化系统疾病

• 发表时间: 2010
• 作者: Sato T;Takimoto R;小池和彦(共著)
• 通讯作者: 小池和彦(共著)

Molecular alterations, the cellular phenotype and proliferation in Barrett's esophagus in a Japanese population with H. pylori infection

幽门螺杆菌感染的日本人群巴雷特食管的分子改变、细胞表型和增殖

• 发表时间: 2010
• 作者: Watari J;Moriichi K;Tanabe H;et. al.
• 通讯作者: et. al.

A pseudosarcomatous lesion resembling a malignant tumor of the esophagocardiac junction, diagnosed by a total biopsy with endoscopic surgery

类似食管贲门交界处恶性肿瘤的假肉瘤病变，通过内窥镜手术进行全活检诊断

• DOI: 10.1055/s-0031-1291502
• 发表时间: 2012
• 期刊: Endoscopy
• 影响因子: 9.3
• 作者: Ando K;Fujiya M;Ito T;Watari J;et al
• 通讯作者: et al

Autofluorescence imaging and the quantitative intensity of fluorescence for evaluating the dysplastic grade of colonic neoplasms

自发荧光成像和荧光定量强度评估结肠肿瘤的发育不良级别

• 发表时间: 2012
• 期刊: Int J Colorectal Diseases
• 作者: Moriichi K;Fujiya M;et al.
• 通讯作者: et al.

わが国のGERDとバレット食道の関わり

日本GERD与Barrett食管的关系

• 发表时间: 2012
• 期刊: THEGIFOREFRONT
• 作者: 渡二郎;富田寿彦;大島忠之;他
• 通讯作者: 他