Analysis about the factors that affect the postnatal formationand function of pancreatic β-cell in connection with the intrauterine undernutrition.

宫内营养不良影响出生后胰岛β细胞形成和功能的因素分析

• 批准号: 22590976
• 负责人: TOYODA Kentaro
• 金额: $ 2.91万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590976/
• 关键词: エネルギー; 糖質代謝異常; イメージング; 糖尿病; 糖代謝異常; 膵島

The aim of this study is to analyze the factors that affect the postnatal formation of pancreatic β-cell mass and function in connection with the intrauterine undernutrition. Although, pregnant mice were divided into two groups; calorie restriction group and control diet group, an individual difference of the daily intake volume severely large unexpectedly. Therefore, in the period of this project, we could not compare with both groups. For detail observation of the change in pancreatic β-cell mass during embryonic and postnatal period, we analyzed the pregnant mice and fetal mice whose pancreatic β-cell express luciferase by using IVIS system and optical projection tomography (OPT). Luciferine injection enabled us to observe the mass of fetal islets in the pregnant mice by IVIS and the number and volume of fetal islet were quantified by OPT analysis.

本研究的目的是分析与宫内营养不良有关的影响出生后胰腺β细胞群形成和功能的因素。虽然将妊娠小鼠分为两组;热量限制组和对照饮食组，但出乎意料的是，每日摄入量的个体差异非常大。因此，在本项目期间，我们无法与两组进行比较。为了详细观察胚胎期和出生后胰腺β细胞群的变化，我们采用IVIS系统和光学投影断层扫描（optical projection tomography，OPT）技术对胰腺β细胞表达荧光素酶的孕鼠和胎鼠进行了分析。用IVIS法观察孕鼠胚胎胰岛的质量，用OPT法定量分析胚胎胰岛的数量和体积。

项目成果

Synthesis and evaluation of radioiodinated exendin derivatives for the imaging of GLP-1 receptor expression in pancreatic islets.

用于胰岛 GLP-1 受体表达成像的放射性碘化毒蜥外泌肽衍生物的合成和评估。

• 发表时间: 2010
• 作者: Kimura H;Ogawa Y;Kawashima H;Mukai E;Toyoda K;Fujimoto H;Hirao K;Ono M;Inagaki N;Saji H.
• 通讯作者: Saji H.

Current Status of Clinical Pancreas and Islet Transplantation in Japan.

日本临床胰腺和胰岛移植的现状。

• 发表时间: 2012
• 作者: Toyada K;Iwanaga Y;Kawaguchi M;Uemoto S;Inagaki N.
• 通讯作者: Inagaki N.

Radiosynthesis and evaluation of F-18 Exendin-4 derivatives as a PET tracer for the imaging of GLP-1receptors on pancreaticβ-cells

F-18 Exendin-4 衍生物作为 PET 示踪剂用于胰腺β细胞上 GLP-1 受体成像的放射合成和评估

• 发表时间: 2011
• 作者: Kimura H;Ogawa Y;Kawashima H;Toyoda K;Mukai E;Fujimoto H;Ono M;Hirao K;Inagaki N;Saji H.
• 通讯作者: Saji H.

Three dimensional ex vivo imaging and analysis of intraportal islet transplants.

门静脉内胰岛移植的三维离体成像和分析。

• 发表时间: 2011
• 期刊: Transpl. Int.
• 作者: Fujimoto;H.;Toyoda;K.;Inagaki N
• 通讯作者: Inagaki N

膵β細胞GLP-1受容体標的放射性プローブとしてのI-123標識Exendin-4誘導体

I-123标记的Exendin-4衍生物作为靶向胰腺β细胞GLP-1受体的放射性探针

• 发表时间: 2011
• 作者: 小川祐;木村寛之;河嶋秀和;豊田健太郎;向英里;藤本裕之;平尾佳;松田洋和;小野正博;稲垣暢也;佐治英郎
• 通讯作者: 佐治英郎