Pathogen moleculoar pattern recognition mechanisms inside and outsied of the blood vessel

血管内外病原体分子模式识别机制

• 批准号: 22591064
• 负责人: FUKUDOME Kenji
• 金额: $ 2.83万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591064/
• 关键词: 血液免疫学; モノクローナル抗体; 粗面小胞体; ストレスシグナル; TLR4; TLR; 血管内皮; 敗血症; アゴニスト; 抗体

We demonstrated that sensitive LPS-recogition by macrophadese was due to the strong TLR4-signal transduction induced by raft-formation in the cells carring memebrane-associated CD14. The signal transduction was not so effective in CD14-negative cells such as B cells; however, it was significant to induce some responses. We discovered that LPS-tolerance was resulted by loss of antigen-presenting function in B cells differenciated by LPS-stimulation in the absence of antigen.

我们证明巨噬细胞对LPS的敏感识别是由于携带膜相关CD14的细胞中筏形成诱导的强TLR4信号转导。在CD14阴性细胞如B细胞中，信号转导不是那么有效，但是，它对诱导一些反应是有意义的。我们发现，LPS耐受是由于在缺乏抗原的情况下，LPS刺激分化的B细胞丧失抗原提呈功能所致。

项目成果

Endotoxin tolerance attenuates airway allergic inflammation in model mice by suppression of the T-cell stimulatory effect of dendritic cells

• DOI: 10.1093/intimm/dxq062
• 发表时间: 2010-09-01
• 期刊: INTERNATIONAL IMMUNOLOGY
• 影响因子: 4.4
• 作者: Matsushita, Hidetomo;Ohta, Shoichiro;Izuhara, Kenji
• 通讯作者: Izuhara, Kenji

Blocking endothelial protein C receptor (EPCR) accelerates thrombus development in vivo

• DOI: 10.1160/th09-11-0750
• 发表时间: 2010-06-01
• 期刊: THROMBOSIS AND HAEMOSTASIS
• 影响因子: 6.7
• 作者: Centelles, Miguel N.;Puy, Cristina;Hermida, Jose
• 通讯作者: Hermida, Jose

Inhibition of antibody production in vivo by pre-stimulation of Toll-like receptor 4 before antigen priming is caused by defective B cell priming and not impairment in antigen presentation.

在抗原引发前预刺激 Toll 样受体 4 抑制体内抗体产生是由 B 细胞引发缺陷引起的，而不是抗原呈递受损所致。

• DOI: 10.1093/intimm/dxs096
• 发表时间: 2013
• 期刊: International Immunology
• 影响因子: 4.4
• 作者: Nurlaely Mida Rachmawati;Kenji Fukudome;Naoko Tsuneyoshi;Uleng Bahrun;Hiroki Tsukamoto;Tsutomu Yanagibashi;Yoshinori Nagai;Kiyoshi Takatsu;Shoichiro Ohta;and Masao Kimoto
• 通讯作者: and Masao Kimoto

Agonistic anti-TLR4 monoclonal antibodies function as immunoadjuvants.

激动性抗 TLR4 单克隆抗体起到免疫佐剂的作用。

• 发表时间: 2010
• 作者: Tsukamoto H;Fukudome K;Takao S;Tsuneyoshi N;Kimoto M.;加藤光次;Rachmawati NM
• 通讯作者: Rachmawati NM

Activated protein C utilizes the angiopoietin/Tie2 axis to promote endothelial barrier function

• DOI: 10.1096/fj.09-134445
• 发表时间: 2010-03-01
• 期刊: FASEB JOURNAL
• 影响因子: 4.8
• 作者: Minhas, Nikita;Xue, Meilang;Jackson, Christopher J.
• 通讯作者: Jackson, Christopher J.