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PET-MRI analysis of cerebral glucose metabolism of rat acute diffuse brain injury.

急性弥漫性脑损伤大鼠脑糖代谢的PET-MRI分析

基本信息

批准号：
22591585
负责人：
FUJINAKA Toshiyuki
金额：
$ 2.75万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591585/
关键词：
頭部外傷学びまん性脳損傷 18fluorodeoxyglucose PET-MRI 18flourodeoxyglucose PRT-MRI

项目摘要

Background and Purpose:Diffuse brain injury is characterized by major cerebral dysfunction despite the mild destruction of brain tissue. There is no report about the continuous change of cerebral glucose metabolism from the very acute to the subacute phase of head trauma. Clinically, it may be impossible to assess the continuous cerebral glucose metabolism with using PET at the acute stage of head trauma. The purpose of this study is to reveal cerebral glucose metabolic change of rats in the acute stage of diffuse brain injury with using PET -MRI that can simultaneously image the MRI and PET.Method: We used 18F-fluorodeoxyglucose(FDG), as the PET tracer. We divided the rat into two groups. One was the trauma group to add the trauma, ant the other was the non-trauma group which was taken of anesthesia and skin incision in a similar way of the trauma group but did not be added of impact. In this study, we used a model of diffuse braininjury reported by Marmarou (Marmarou et.al, 1994). We … More added an impact to the head of rats with the weight of 500g, which was fallen down above a metal plate fixed to the skull of the rat. We can evenly add an impact and can create a diffuse brain injury model. PET -MRI was taken before the procedure in all rats, and 3 hours, 1 day, 4 days, and 7days after the procedure. We got images of PET -MRI by all rats, and compared the trauma group with non-trauma group. Result:At 3 hours and 1 day after the procedure, cerebral glucose metabolism in traumatic group was inclined to reduce compared to the non-traumatic group, but there is no statistically significant difference(3 hours:p=0.876、day1:p=0.947). At the 4days after the procedure, cerebral glucose metabolism in the trauma group was inclined to increase compared to the non-trauma group, but there was also no statistically significant difference(p=0.215). At 7 days after the procedure, glucose metabolism was significantly increased in the trauma group(p=0.035). The present study revealed that cerebral glucose metabolism tends to be suppressed in ultra-acute phase of diffuse brain injury , and that it tends to increase excessively in the subacute phase. Less

背景与目的：弥漫性脑损伤的特点是尽管脑组织有轻度破坏，但仍存在严重的脑功能障碍。目前尚无关于颅脑外伤从极急性期到亚急性期脑葡萄糖代谢持续变化的报道。临床上，在头部外伤急性期使用PET可能无法评估持续的脑葡萄糖代谢。本研究的目的是利用可同时进行MRI和PET成像的PET-MRI来揭示大鼠弥漫性脑损伤急性期的脑葡萄糖代谢变化。方法：采用18F-氟脱氧葡萄糖(FDG)作为PET示踪剂。我们将大鼠分为两组。一种是创伤组，增加创伤；另一种是非创伤组，采用与创伤组类似的方式进行麻醉和切皮，但不增加冲击力。在这项研究中，我们使用了 Marmarou 报道的弥漫性脑损伤模型（Marmarou 等，1994）。我们在老鼠的头部添加了一个重达 500 克的冲击力，该冲击力落在固定在老鼠头骨上的金属板上。我们可以均匀地添加影响并创建弥漫性脑损伤模型。所有大鼠在手术前以及手术后3小时、1天、4天和7天进行PET-MRI。对所有大鼠进行PET-MRI检查，并将创伤组与非创伤组进行比较。结果：术后3小时和第1天，创伤组脑糖代谢较非创伤组有降低趋势，但差异无统计学意义（3小时：p=0.876、第1天：p=0.947）。术后第4天，创伤组脑糖代谢较非创伤组有升高趋势，但差异无统计学意义（p=0.215）。术后7天，创伤组的葡萄糖代谢显着增加（p=0.035）。本研究表明，在弥漫性脑损伤的超急性期，脑葡萄糖代谢趋于受到抑制，而在亚急性期，脑葡萄糖代谢趋于过度增加。较少的