Regeneration of Damaged Peripheral Nerve using Skeletal Muscle-Derived Multipotent Stem Cell co-using with Absorbable Tube

为了开发不可逆长神经缺损的新疗法，我们应用骨骼肌来源的多能干细胞片颗粒（Sk-MSCSPs）作为一种新的替代神经自体移植来源。我们将导管与细胞化导管（用70%乙醇脱水食管粘膜）连接。4周后，导管被GFP+细胞/组织填充，移植的GFP+供体细胞/组织明显向后伸展至受体神经近端。8周后，上述性能进一步增强。这些结果强烈提示Sk-MSCSPs可能是长神经间隙治疗最合适的干细胞。因此，我们希望以临床应用为目标，继续深入研究。

To develop the new therapy for the irreversible long nerve deficit, we applied skeletal muscle-derived multipotent stem cell sheet pellets (Sk-MSCSPs), as a novel alternative source of nerve autograft. We used the tubeto the dcellularized conduit (dehydrated esophageal submucous membrane by 70% ethanol). After 4 weeks, conduit was filed by GFP+ cells/tissues, and engrafted GFP+ donor cells/tissues obviously stretch back to proximal portion of recipient nerve. After 8 weeks, theabove properties were more enhanced. These results strongly suggested that Sk-MSCSPs may be the best suitable stem cells for the long nerve gap therapy. Therefore, we want to continue further study with the aim of clinical application.