The role of androgen on brain protection by inhalation anestheticsduring cerebral reperfusion

雄激素对脑再灌注时吸入麻醉药脑保护的作用

基本信息

批准号：
22591705
负责人：
UCHIDA Masayoshi
金额：
$ 2.58万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

The diagnosis of perioperative cerebral ischemia is difficult, but the onset of cerebral infarction is fatal. Thus, the prevention of such event is very important for anestheticmanagement. Gender differences are observed in the incidence of cerebral infarction, and generally the incidence of cerebral infarction is less in females than males. It is suggested that the estrogen is strongly involved in this mechanism. On the other hand, there is little information about the role of androgen regarding ischemic events. It hasbeen reported that neuroprotective effect through preconditioning induced by inhalation anesthetic was found only in males, and not seen in females. There is a possibility that the gender difference is involved in the protective effect of inhaled anesthetic for the brain ischemia. Then we intended to investigate the interaction between androgen and sevoflurane regarding cerebral protective effect. Firstly, in order to evaluate thecerebral endothelial function following i … More schemia/reperfusion, we used the closed cranial window preparation associated with the middle cerebral artery occlusion model in the Sprague-Dawley rat. To evaluate the cerebrovascular endothelial function before and after cerebral ischemia/reperfusion, topical administration of acetylcholine could be applied.Unfortunately, by the inhalation of sevoflurane, the attenuation effect of vascular endothelial dysfunction caused by ischemia/reperfusion was not observed.Additionally, we evaluated the tissue oxygen partial pressure of spinal cord (PsptO2) following aortic cross-clamping and -unclamping. In anesthetized rabbits, we prepared for measurement of PsptO2 following aortic cross-clamping using tissue PO2 monitoring. In the experiment, we allocated 18 animals into 3 groups,(d) control group; n=6,(e) estrogen (17β estradiol: 20μg) group; n=6,(f) fulvestrant pretreatment (1mg/kg) groupn=6. Administration of 17β estradiol improved deterioration of the values in PsptO2 at 20 min after aortic cross-clamping and 0, 2, 5 min after aortic unclamp. The improvement was diminished by pretreatment of fulvestrant. Less

周期性脑缺血的诊断很困难，但是大脑梗死的发作是致命的。那就预防这种事件对于麻醉管理非常重要。在脑梗塞的发生率中观察到性别差异，通常，女性的脑梗塞发生率小于男性。建议雌激素强烈参与该机制。另一方面，关于雄激素对缺血事件的作用的信息很少。据报道，仅在男性中发现了通过吸入麻醉引起的预处理的神经保护作用，而在女性中没有发现。性别差异可能与遗传性麻醉对脑缺血的保护作用有关。然后，我们打算研究雄激素和七氟醚在脑受保护作用方面的相互作用。首先，为了评估I…更多的案例/再灌注后的大脑内皮功能，我们使用了Sprague-Dawley大鼠中与中大脑中动脉闭塞模型相关的闭合颅窗制剂。为了评估脑缺血/再灌注之前和之后的脑血管内皮功能，可以应用乙酰胆碱的局部给药。主动脉交叉夹和 - 无障碍后，脊髓（PSPTO2）。在麻醉的兔子中，我们准备使用组织PO2监测的主动脉交叉联系后的PSPTO2测量。在实验中，我们将18只动物分为3组，（d）对照组。 n = 6，（E）雌激素（17β雌二醇：20μg）组； n = 6，（f）Fulvestort prestrant（1mg/kg）groupN = 6。主动脉交叉钳位后20分钟，在主动脉脱落后的0、2、5分钟后，施用17β雌二醇改善了PSPTO2中值的定义。预处理预处理减少了改善。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of intravenous anesthetics and sedatives on mucin production in cultured human airway epithelial cells

静脉麻醉药和镇静剂对培养的人气道上皮细胞粘蛋白产生的影响

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
SugiyamaY;Tanabe K;Iida H.
通讯作者：
Iida H.

Involvement of phosphatidylinosiol 3-kinase/Akt on basic fibroblast growth factor-induced glial cell line-derived neurotrophic factor release from rat glioma cells.

磷脂酰肌醇 3-激酶/Akt 参与碱性成纤维细胞生长因子诱导的大鼠神经胶质瘤细胞释放神经胶质细胞系源性神经营养因子。

DOI：
10.1016/j.brainres.2012.04.057
发表时间：
2012
期刊：
Brain Reseach.
影响因子：
0
作者：
溝田敏幸;辻川洋;正田丈裕;福田和彦;Takeda J.;Tanabe K
通讯作者：
Tanabe K

麻酔前の評価・準備と予後予測―病態に応じた周術期管理のために．

麻醉前评估/准备和预后预测——根据病理情况进行围手术期管理。

DOI：
发表时间：
2012
期刊：
影响因子：
0
作者：
Zhi-hong Lu;Satoru Fukuda;Yoichi Minakawa;Atsushi Yasuda;Hidetoshi Sakamoto;Shigehito Sawamura;Hidenori Takahashia;Noriko Ishii;川口昌彦;井上聡己;飯田宏樹
通讯作者：
飯田宏樹

Nicorandil protects pial arterioles from endothelial dysfunction induced by smoking in rats.

尼可地尔可保护大鼠软脑膜小动脉免受吸烟引起的内皮功能障碍的影响。