Combined therapy for urologic cancer cells by high-intensity focused ultrasound and PEG-conjugated fullerene

通过聚乙二醇化修饰富勒烯(C60)，成功地合成了水溶性的聚乙二醇偶联富勒烯。MTS分析表明，HIFU与聚乙二醇偶联富勒烯在体外对PC-3细胞有协同生长抑制作用。然而，在体内，由于标记的聚乙二醇偶联富勒烯的不稳定性，我们未能确认富勒烯选择性肿瘤组织聚集的结果的重复性。在鸡组织模型中，我们发现了这种联合治疗的协同组织退变效应，而在PC-3异种移植模型中没有表现出相加或协同的抗肿瘤作用。

By chemically modifying fullerene (C60) with polyethylene glycol (PEG), we succeeded in synthesizing water-soluble PEG-conjugated fullerene. MTS assays showed that thecombination of HIFU with PEG-conjugated fullerene resulted in synergistic growth inhibitory effects in PC-3 cells in vitro. In vivo, however, we failed to confirm reproducibility on the results of selective tumor tissue accumulation of fullerene because of instability of labeled PEG-conjugated fullerene. Using chicken tissue model we found synergistic tissue degenerating effects by this combination therapy, which showed no additive or synergistic antitumor effects in PC-3 xenograft models.