Combined therapy for urologic cancer cells by high-intensity focused ultrasound and PEG-conjugated fullerene

用聚乙二醇（PEG）对富勒烯（C60）进行化学修饰，成功地合成了水溶性的聚乙二醇共轭富勒烯。MTS实验显示，HIFU与peg共轭富勒烯联合使用对体外PC-3细胞具有协同生长抑制作用。然而，在体内，由于标记的peg共轭富勒烯的不稳定性，我们未能证实选择性肿瘤组织积累富勒烯结果的可重复性。在鸡组织模型中，我们发现该联合治疗对组织变性有协同作用，而对PC-3异种移植瘤模型无加性或协同抗肿瘤作用。

By chemically modifying fullerene (C60) with polyethylene glycol (PEG), we succeeded in synthesizing water-soluble PEG-conjugated fullerene. MTS assays showed that thecombination of HIFU with PEG-conjugated fullerene resulted in synergistic growth inhibitory effects in PC-3 cells in vitro. In vivo, however, we failed to confirm reproducibility on the results of selective tumor tissue accumulation of fullerene because of instability of labeled PEG-conjugated fullerene. Using chicken tissue model we found synergistic tissue degenerating effects by this combination therapy, which showed no additive or synergistic antitumor effects in PC-3 xenograft models.