New therapeutic strategy of pediatric surgical treatment for repair of tissue deficits and revascularization using autologous tissue grafting

我们利用脐带血内皮祖细胞和脐带华氏胶间充质干细胞，利用光刻技术建立了具有毛细血管网络的羊膜片。与对照组相比，将带毛细血管网络的羊膜片植入缺血小鼠后肢后，再灌注增加。自体细胞体外工程的毛细血管网络的植入可能具有治疗小儿外科的潜力。

We established amnion sheets with capillary network by use of lithographic technique using outgrowth endothelial progenitor cells from umbilical cord blood and mesenchymal stem cells from Wharton’s jelly in umbilical cord. Implantation of the amnion sheet with capillary network into the ischemic hind limbs of mice increased reperfusion compared with controls. Implantation of a capillary network engineered ex vivo from autologous cells may have therapeutic potential for the treatment of pediatric surgery.