The pathogenesis induced by highly pathogenic avian influenza virus H5N1 hemagglutinin

高致病性禽流感病毒H5N1血凝素诱发的发病机制

• 批准号: 22790953
• 负责人: DAIDOJI Tomo
• 金额: $ 2.58万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790953/
• 关键词: 高病原性鳥インフルエンザ; 高病原性インフルエンザウイルス; アポトーシス

In recent years, the highly pathogenic avian influenza virus H5N1 has raised serious worldwide concern about an influenza pandemic ; however, the mechanism of H5N1 pathogenesis is largely unknown. A/ Crow/ Kyoto/ 53/ 2004(H5N1)[ Cr/ Ky(H5N1)] showed high mortality and weight loss toward mice, while A/ Duck/ Hong Kong/ 820/ 80(H5N3) did not. In addition, recombinant H5N3 viruses carrying the H5N1 HA gene(rH5N3H5N1HA) showed the similar result with that of Cr/ Ky(H5N1). In addition, Cr/ Ky(H5N1) and rH5N3H5N1HA also indicated efficient viral replication and lung hemorrhage including inflammatory cell infiltration. The levels of proinflammatory cytokine in mice infected with rH5N3H5N1HA was higher than mice infected with Dk/ Hk(H5N3). Taken together, these data suggest that H5N1HA broaden the viral pathogenesis in mice and this pathogenesis is based on viral replication and proinflammatory cytokine balance in mice.

近年来，高致病性禽流感病毒H5N1引起了世界范围内对流感大流行的严重关注，但H5N1的致病机制在很大程度上尚不清楚。A/Crow/京都/53/2004(H5N1)[Cr/Ky(H5N1)]对小鼠有较高的死亡率和体重减轻，而A/Duck/Hong Kong/820/80(H5N3)则没有。此外，携带H5N1 HA基因的重组H5N3病毒(RH5N3H5N1HA)与CrKy(H5N1)的结果相似。此外，Cr/Ky(H5N1)和rH5N3H5N1HA也提示了病毒的有效复制和肺出血，包括炎细胞浸润。RH5N3H5N1HA感染小鼠的促炎细胞因子水平高于DK/HK(H5N3)感染小鼠。综上所述，这些数据表明，H5N1HA扩大了小鼠的病毒致病机制，这种致病机制是基于病毒复制和小鼠的促炎细胞因子平衡。

项目成果

The role of apoptosis in influenza virus pathogenesis and the mechanisms involved in anti-influenza therapies

细胞凋亡在流感病毒发病机制中的作用以及抗流感治疗的机制

• 发表时间: 2010
• 期刊: Current Chemical Biology
• 作者: Daidoji T;Kaihatsu K;Nakaya T
• 通讯作者: Nakaya T

Novel Type II Transmembrane Serine Proteases, MSPL and TMPRSS13, Proteolytically Activate Membrane Fusion Activity of the Hemagglutinin of Highly Pathogenic Avian Influenza Viruses and Induce Their Multicycle Replication

• DOI: 10.1128/jvi.02605-09
• 发表时间: 2010-05-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Okumura, Yuushi;Takahashi, Etsuhisa;Kido, Hiroshi
• 通讯作者: Kido, Hiroshi

Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 2010.

• DOI: 10.1371/journal.pone.0030946
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Yasugi M;Nakamura S;Daidoji T;Kawashita N;Ramadhany R;Yang CS;Yasunaga T;Iida T;Horii T;Ikuta K;Takahashi K;Nakaya T
• 通讯作者: Nakaya T

高病原性鳥インフルエンザウイルスH5N1はヒト呼吸器上皮細胞に対して広いトロピズムを示す

高致病性禽流感病毒H5N1对人呼吸道上皮细胞表现出广泛的趋向性

• 发表时间: 2012
• 作者: 大道寺智;Ibrahim M.S.;渡辺洋平;安木真世;丸山央峰;増田泰輔;新井史人;大場誠介;本田文江;生田和良;中屋隆明
• 通讯作者: 中屋隆明

Novel type II transmembrane serine proteases, MSPL and TMPRSS13

新型 II 型跨膜丝氨酸蛋白酶、MSPL 和 TMPRSS13

• 发表时间: 2010
• 期刊: Proteolytically activate membrane fusion activity of the hemagglutinin of highly pathogenic avian influenza viruses and induce their multicycle replication
• 作者: Okumura Y;Takahashi E;Yano M;Ohuchi M;Daidoji T;Nakaya T;Bottcher E;Garten W;Klenk HD;Kido H
• 通讯作者: Kido H