Structural determinant of Humanβ-defensin-3 to stimulate cytokine/chemokine secretion

人β-防御素-3（HBD-3）具有刺激促炎细胞因子/趋化因子分泌的能力，其连接先天性和适应性免疫以及抗微生物活性。虽然我们假设N端的α-螺旋是这一功能的结构决定因素，并研究了对正常人表皮角质形成细胞分泌IL-6和IL-8的影响，但IL-6和IL-8的分泌与HBD-3 C端独特模体中的两个Arg残基有关，而与α-螺旋无关。

Humanβ-defensin-3(HBD-3) possesses an ability to stimulate proinflammatory cytokine/chemokine secretion, which links innate and adaptive immunity, as well as antimicrobial activity. Although we hypothesized thatα-helics in N-terminal is a structural determinant for this function and examined the effect on normal human epidermal keratinocytes to secrete IL-6 and-8, the secretion of IL-6 and-8 was associated with two Arg residues in C-terminal unique motif of HBD-3 not withα-helics.