Structural determinant of Humanβ-defensin-3 to stimulate cytokine/chemokine secretion

Humanβ-defensin-3 刺激细胞因子/趋化因子分泌的结构决定因素

• 批准号: 22791075
• 负责人: SHIRAFUJI Yoshinori
• 金额: $ 2.5万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791075/
• 关键词: ヒトβデフェンシン-3; αヘリカル; アルギニン; IL-6; IL-8; デフェンシン; αヘリクス; サイトカイン

Humanβ-defensin-3(HBD-3) possesses an ability to stimulate proinflammatory cytokine/chemokine secretion, which links innate and adaptive immunity, as well as antimicrobial activity. Although we hypothesized thatα-helics in N-terminal is a structural determinant for this function and examined the effect on normal human epidermal keratinocytes to secrete IL-6 and-8, the secretion of IL-6 and-8 was associated with two Arg residues in C-terminal unique motif of HBD-3 not withα-helics.

人β-防御素-3（HBD-3）具有刺激促炎细胞因子/趋化因子分泌的能力，其连接先天性和适应性免疫以及抗微生物活性。虽然我们假设N端的α-螺旋是这一功能的结构决定因素，并研究了对正常人表皮角质形成细胞分泌IL-6和IL-8的影响，但IL-6和IL-8的分泌与HBD-3 C端独特模体中的两个Arg残基有关，而与α-螺旋无关。

项目成果

Elevated expression of Paneth cell CRS4C in ileitis-prone SAMP1/YitFc mice: regional distribution, subcellular localization, and mechanism of action.

易患回肠炎的 SAMP1/YitFc 小鼠中潘氏细胞 CRS4C 表达升高：区域分布、亚细胞定位和作用机制。

• DOI: 10.1074/jbc.m109.083220
• 发表时间: 2010
• 期刊: The Journal of biological chemistry
• 作者: Shanahan,MichaelT;Vidrich,Alda;Shirafuji,Yoshinori;Dubois,ClaireL;Henschen-Edman,Agnes;Hagen,SusanJ;Cohn,StevenM;Ouellette,AndréJ
• 通讯作者: Ouellette,AndréJ

Two Arg in C-terminal of human beta-defensin-3 are essential for the stimulation of proinflammatory cytokine secretion in keratinocytes

人β-防御素-3 C 末端的两个精氨酸对于刺激角质形成细胞中促炎细胞因子的分泌至关重要

• 发表时间: 2011
• 作者: Shirafuji Y;Yasui Y;Morizane S;Iwatsuki K
• 通讯作者: Iwatsuki K

Elevated expression of paneth cell CRS4C in ileitis-prone sampl/YitFc mice : Regional distribution, subcellular localization, and mechanism of action.

易患回肠炎的样本/YitFc 小鼠中潘氏细胞 CRS4C 表达升高：区域分布、亚细胞定位和作用机制。

• 发表时间: 2010
• 期刊: J Biol Chem
• 影响因子: 4.8
• 作者: Shanahan MT;Vidrich A;Shirafuji Y;Dubois CL;Henschen-Edman A;Hagen SJ;Cohn SM;Ouellette AJ
• 通讯作者: Ouellette AJ

Two Arginine residues of C-terminal are essential for the unique salt-insensitive and broad antimicrobial activities of human beta-defensin-3

C 末端的两个精氨酸残基对于人类 β-defensin-3 独特的盐不敏感和广泛的抗菌活性至关重要

• 发表时间: 2010
• 作者: Yoko Yasui;Yoshinori Shirafuji;Keiji Iwatsuki
• 通讯作者: Keiji Iwatsuki