Determinants of antimicrobial activity and salt-resistance of defensin

防御素抗菌活性和耐盐性的决定因素

• 批准号: 20790803
• 负责人: SHIRAFUJI Yoshinori
• 金额: $ 2.58万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790803/
• 关键词: デフェンシン; アルギニン; 抗菌活性; 塩抵抗性; 黄色ブドウ球菌; ヒトβデフェンシン-3; 黄色ブドウ球菌(4)緑膿菌; 塩抵抗性(6)MRSA; メタロβラクタマーゼ産生緑膿菌

Human β-defensin (HBD) -3 has potent and salt-resistant antimicrobial activity against both gram-negativeand gram positive bacteria and this activity, while other HBDs possess antimicrobial activity against only gram-negative strains and it is attenuated under salt existence. We hypothesized that these characteristics are due to two arginine residues in the COOH-terminal of HBD-3 which is not involved in other HBD-3. To investigate, we designated two recombinant mutants of HBD-3; desR HBD-3 in which these two arginine residues were deleted from HBD-3 and NRR-HBD-3 in which these two arginine residues were shifted from COOH-terminal to NH2-terminal and esxamined their antimicrobial activities and salt-resistance. The results showed that these two argine residues are required to exist in the COOH-terminal in order to maintain the activity and salt-resistance of HBD-3.

人β防御素(HBD)-3对革兰氏阴性菌和革兰氏阳性菌均有较强的抗盐抗菌活性，而其他HBD只对革兰氏阴性菌有抗菌活性，在盐存在下其抗菌活性减弱。我们推测，这些特征是由于HBD-3的COOH-末端有两个精氨酸残基，而其他HBD-3没有涉及到。为了进行研究，我们命名了HBD-3的两个重组突变体：Desr HBD-3，其中这两个精氨酸残基从HBD-3中删除，NRR-HBD-3中，这两个精氨酸残基从COOH端移动到NH2端，并对它们的抗菌活性和耐盐性进行了研究。结果表明，为了保持HBD-3的活性和耐盐性，这两个精氨酸残基需要存在于COOH-末端。

项目成果

Elevated expression of Paneth cell CRS4C in ileitis-prone SAMP1/YitFc mice: regional distribution, subcellular localization, and mechanism of action.

易患回肠炎的 SAMP1/YitFc 小鼠中潘氏细胞 CRS4C 表达升高：区域分布、亚细胞定位和作用机制。

• DOI: 10.1074/jbc.m109.083220
• 发表时间: 2010
• 期刊: The Journal of biological chemistry
• 作者: Shanahan,MichaelT;Vidrich,Alda;Shirafuji,Yoshinori;Dubois,ClaireL;Henschen-Edman,Agnes;Hagen,SusanJ;Cohn,StevenM;Ouellette,AndréJ
• 通讯作者: Ouellette,AndréJ

アトピー性皮膚炎病変部に浸潤する炎症細胞による抗菌ペプチドの発現

浸润特应性皮炎皮损的炎症细胞表达抗菌肽

• 发表时间: 2008
• 作者: Yoshinori Shirafuji;Yoko Sakagami;Takashi Oono;Keiji Iwatsuki;Yoshinori Shirafuji;大野貴司
• 通讯作者: 大野貴司

デフェンシン活性化のメカニズム

防御素激活机制

• 发表时间: 2008
• 作者: Yoko Sakagami;Yoshinori Shirafuji;Takahi Oono;Keiji Iwatsuki;白藤宜紀
• 通讯作者: 白藤宜紀

Antimicrobial activity of human beta-defensin-3 against metallo beta lactamase producing Pseudomonas

人β-防御素-3对产生金属β内酰胺酶的假单胞菌的抗菌活性

• 发表时间: 2008
• 作者: Yoshinori Shirafuji;Yoko Sakagami;Takashi Oono;Keiji Iwatsuki
• 通讯作者: Keiji Iwatsuki

Two Arginine residues in the COOH-terminal of human beta-defensin-3 affect broad antimicrobial activities and salt-resistance

人 β-防御素 3 COOH 末端的两个精氨酸残基影响广泛的抗菌活性和耐盐性

• 发表时间: 2009
• 作者: Shanahan;M. T、. Vidrich;A. 、Shirafuji;Y.;他5名;阪上陽子
• 通讯作者: 阪上陽子