Molecular mechanisms and effects of X-ray and particle beam for invasiveness in glioblastoma cell

用X射线或碳离子束照射人胶质母细胞瘤细胞系。不仅是X射线，而且碳离子束在照射后24小时增加了细胞的活力。阻断.-amino-3-hydroxy-5-methyl-4-isoxazolepropionic酸型谷氨酸受体可抑制胶质母细胞瘤细胞的迁移。照射和谷氨酸受体拮抗剂可改变E-和N-钙粘素蛋白的表达。这些结果提示谷氨酸和钙粘附素可能在辐射促进胶质母细胞瘤细胞运动中起作用。此外，谷氨酸受体拮抗剂可能是胶质母细胞瘤放射治疗中有用的细胞迁移抑制剂。

Human glioblastoma cell lines were irradiated with X-rays or carbon ion beams. Not only X-rays but carbon ion beams increased cell motility at 24 hours after irradiation. Blockage of .-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptor suppressed migration in glioblastoma cells. Expression of E-and N-cadherin proteins was changed by irradiation and glutamate receptor antagonist. These results suggest that glutamate and cadherin might play a role in facilitation of glioblastoma cell motility by irradiation. And also, glutamate receptor antagonist may useful inhibitor for cell migration in radiation therapy of glioblastoma.