Development of high sensitive MRI contrast agent encapsulating CEST agent into apoferritin cavity

脱铁铁蛋白腔内封装CEST剂的高灵敏度MRI造影剂的研制

• 批准号: 22791187
• 负责人: MAKINO Akira
• 金额: $ 2.5万
• 依托单位: Kyoto University (2011)Shimadzu Corporation (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791187/
• 关键词: 核磁気共鳴画像; CEST; アポフェリチン

Lanthanoid complexes having various electric charges were newly designed and synthesized as new chemical exchange and saturation transfer(CEST) agents. The complexes were encapsulated into apoferritin cavity, and thier character as CEST type MRI contrast agent was evaluated in vitro. Further, surface of apoferritin is modified with hydrophilic polymers to extend its blood circulation behavior, and I succeeded to deliver the dextran-coated apoferritin to transplanted tumor region utilizing enhanced permeation and retention(EPR) effect. Performance of the apoferritin MRI contrast agent encapsulating CEST agent in vivo was examined using tumor-transplanted mouse.

设计并合成了具有多种电荷的类镧配合物，作为新型化学交换和饱和转移（CEST）剂。将复合物包埋在铁蛋白腔内，体外评价其作为CEST型MRI造影剂的特性。此外，用亲水性聚合物修饰了载铁蛋白的表面，延长了其血液循环行为，我成功地利用增强的渗透和保留（EPR）效应将右旋糖酐包被的载铁蛋白递送到移植肿瘤区域。采用肿瘤移植小鼠对包封CEST的载铁蛋白MRI造影剂在体内的性能进行了研究。

项目成果

Effective encapsulation of a new cationic gadolinium chelate into apoferritin and its evaluation as an MRI contrast agent

• DOI: 10.1016/j.nano.2011.01.015
• 发表时间: 2011-10-01
• 期刊: NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
• 影响因子: 5.4
• 作者: Makino, Akira;Harada, Hiroshi;Kimura, Shunsaku
• 通讯作者: Kimura, Shunsaku

Preparation of Peptide-and Protein-based Molecular Assemblies and Its Utilization as Nanocarriers for Tumor Imaging

基于肽和蛋白质的分子组装体的制备及其作为肿瘤成像纳米载体的应用

• 发表时间: 2011
• 期刊: React. Funct. Polym
• 作者: Akira Makino;Shunsaku Kimura
• 通讯作者: Shunsaku Kimura

Preparation of Peptide- and Protein-based Molecular Assemblies and Its Utilization as Nanocarriers for Tumor Imaging

基于肽和蛋白质的分子组装体的制备及其作为肿瘤成像纳米载体的应用

• 发表时间: 2011
• 期刊: Reactive and Functional Polymer
• 作者: Y. Uchiyama;H. Fujita;牧野顕
• 通讯作者: 牧野顕